丛枝菌根真菌对丹参木质部结构及防御相关基因的影响  

Effects of Arbuscular mycorrhizal/Fungi on Xylem Structure and Expression of Defense-related Genes in Salvia miltiorrhiza

作　　者：周修腾[1,2] 王雪[1,3] 陈敏[1] 黄璐琦[1] 肖文娟 杨光[1] 李鹏英[1] 华国栋[3] 陈美兰[1] 

机构地区：[1]中国中医科学院中药资源中心/道地药材国家重点实验室培育基地,北京100700 [2]广州中医药大学临床药理研究所,广州510405 [3]北京中医药大学东方医院药学部,北京100078 [4]郫县中医医院,四川郫县611730

出　　处：《中国农学通报》2017年第4期98-104,共7页Chinese Agricultural Science Bulletin

基　　金：国家中医药局行业科研专项经费"30项中药材生产使用技术规范化及其适用性研究"(201407005)

摘　　要：旨在初步探讨丛枝菌根真菌GV对丹参木质结构及合成相关基因PAL、C4H,防御相关基因PR1、WRKY的影响。实验设定对照组和菌根组,测定生长90天后菌根对丹参生长情况及木质部结构的影响,同时采用实时荧光定量的方法测定相关基因PAL、C4H及30、60、75、90、105、130天不同时期PR1、WRKY的表达情况。接种菌根90天丹参根部侵染率为90%,基本达到最大值;地上部分鲜重和叶片数显著高于对照组,约为对照组的2.7倍和1.96倍;根部横切部分的木质部面积,积分光密度显著大于对照组;与对照组相比,地下部分木质素合成基因PAL、C4H表达显著提高,地上部分显著降低;丹参防御基因PR1(地下部分)和WRKY表达量在75天前显著增加,地下部分WRKY表达量在130天显著增加。丛枝菌根真菌能促进丹参生长,增加丹参根部木质部面积以及密度,促进木质素的合成,对提高丹参抗病性有积极作用。To study the effects of GV,an Arbuscular mycorrhizal fungi,on the structure of xylem and synthesis related genes PAL and C4H,defense-related genes PR1,WRKY,the authors set up the control group and mycorrhizal group.After 90 days,the effects of mycorhiza on the Salvia miltiorrhiza growth and the structure of xylem were detected.The expressions of PAL,C4Hand PRIand WRKYwere recorded using real-time PCR at 30,60,75,90,105,and 130 days.The infection rate of S.miltiorrhiza root was 90%,which reached the peak basically.The fresh weight of the above-ground portion and the number of leave in the GV group were significantly higher than that of the control group,about 2.7 and 1.96 times.The integral optical density and area of xylem on root transection of GV group were significantly greater than that of the control group.The expression level of PAL and C4H in the roots was significantly increased in the GV group,while markedly decreased in the above-ground parts.The expression of PR1 in the roots and WRKY in both the roots and above-ground parts increased significantly before 75 days,and the expression of WRKYin roots saw another notable increase at 130 days.Arbuscular mycorrhizal fungi could promote growth,increase the area and density of xylem in the root of S.miltiorrhiza,and play a positive role in its pathogen resistance.

关 键 词：丛枝菌根真菌 丹参 木质素 防御相关基因 

分 类 号：S181[农业科学—农业基础科学]