金铁锁多倍体毛状根的诱导及性质  

Induction and characterization of hairy roots-derived polyploidy of Psammosilene tunicoides

作　　者：施陆 程诺[1] 潘苗苗[1] 陈阳[1] 张宗申[1] 刘同祥 于振艳 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]河南省海乐森药用细胞工程技术有限公司,河南郑州450001

出　　处：《大连工业大学学报》2017年第1期14-18,共5页Journal of Dalian Polytechnic University

基　　金：河南省科技创新杰出人才项目(134200510002)

摘　　要：研究了秋水仙素对金铁锁毛状根多倍化、多倍化后毛状根生长和皂苷积累的变化。结果表明,秋水仙素对金铁锁毛状根的作用效果具有浓度和时间依赖性,其中,经1.25mmol/L的秋水仙素处理24h的金铁锁毛状根根尖存活率最高,达到60%;加倍后细胞核体积明显增加,侧根分支比普通毛状根多,根毛密度也比普通毛状根大。培养35d后的生物量是普通毛状根的1.48倍(干重);多倍体毛状根和普通毛状根在皂苷质量分数方面差别不大,但在同等培养条件下,多倍体毛状根的皂苷总产量较高,达到普通毛状根的1.45倍。结果提示,染色体多倍化是一种提高毛状根质量和皂苷产量的潜在重要技术。The effects of colchicine on polyploidisation of Psammosilene tunicoides hairy roots and changes in both growth of hairy roots and saponins contents were studied. The results showed that the effects of colchicine were in a time and concentration dependent manner, and the maximum survival rate was up to 60% treated by 1.25 mmol/L colchicine for 24 h. The nuclear volume was significantly increased treated by 2.5 mmol/L colchicine, and the colchicine-treated hairy roots had more lateral roots branch and higher roots hair density compared with the normal. The biomass （dry weight） of the treated hairy roots was 1.48 times of that of ordinary hairy roots after cultured for 35 d. The saponin contents of the two types of hairy roots had little differences, but the saponins yield of the treated hairy roots was 1.45 times of that of normal hairy roots under the same culture conditions. The results suggested that polyploidisation of chromosome would be a potential method to improve the efficiency and quality of hairy roots in large scale culture in the future.

关 键 词：金铁锁 毛状根 多倍体 

分 类 号：S567.2[农业科学—中草药栽培]