COPD大鼠肺组织IL-17、IL-21、RORTt的表达及辛伐他汀治疗对其的影响  

Expressions of IL-17,IL-21,RORγt in lung tissue of COPD rats and the effect of simvastatin on it Yuan Lindong

作　　者：袁林栋 王文静[2] 刘晓宇[2] 刘青[7] 孙伯坚[3] 李珠[1] 张彬[4] 姜雪[5] 李鹏[6] 孙武装[1] 王丽红[1] 

机构地区：[1]山东省聊城市人民医院呼吸内科,252000 [2]河北医科大学第一医院呼吸科,石家庄050031 [3]河北医科大学第一医院血液科,石家庄050031 [4]河北医科大学第一医院急诊科,石家庄050031 [5]天津市第三中心医院儿科,300170 [6]浙江省苍南县人民医院内科,温州325800 [7]河北省老年病医院呼吸科,石家庄050031

出　　处：《国际呼吸杂志》2017年第5期330-335,共6页International Journal of Respiration

摘　　要：目的 观察辛伐他汀对吸烟所致COPD大鼠肺组织中IL-17、IL-21及转录因子视黄酸相关孤儿受体γt （retinoid-related orphan nuclear receptor gamma t,RORγt）表达的影响,探讨辛伐他汀治疗COPD的价值及其作用机制.方法 SD雄性大鼠随机分为对照组（10只）、吸烟组（11只）及治疗组（10只）;观察各组大鼠肺组织的病理改变,采用免疫组织化学方法对大鼠肺组织IL-17、IL-21及RORγt蛋白的分布进行检测;Real time RT-PCR方法对肺组织中IL-17、IL-21及RORγtmRNA的表达进行测定.结果 在支气管黏膜上皮和肺泡间隔内,IL-17、IL-21及RORγt阳性细胞在吸烟组[（90.00±14.02） cells/mm2、（61.18±9.16） cells/mm2、（77.27±7.95） cells/mm2]和治疗组[（72.80±10.79） cells/mm2、（49.40±3.34） cells/mm2、（65.40±5.23） cells/mm2]的表达量均高于对照组[（5.20±4.49） cells/mm2、（8.20±3.16） cells/mm2、（6.80±5.85） cells/mm2] （P＜0.01）;且吸烟组的表达量高于治疗组（P＜0.05）.肺组织中IL-17、IL-21及RORγt mRNA的表达吸烟组（7.49±2.93、12.22±4.98、4.04±0.21）和治疗组（2.39±0.62、3.78±0.35、1.76±0.25）均高于对照组（1.00±0.00、1.00±0.00、1.00±0.00）（P＜0.01）,吸烟组的表达量高于治疗组（P＜0.05）.肺泡间隔内的IL-17阳性细胞数与平均内衬间隔呈正相关（r=0.729,P＜0.01）,与平均肺泡数呈负相关（r =-0.889,P＜0.01）.结论 IL-17、IL-21和RORγt在大鼠COPD形成过程中起着一定的作用,辛伐他汀降低大鼠肺内炎症细胞及炎症因子IL-17、IL-21及RORγt的表达,这种作用是辛伐他汀抑制COPD大鼠肺组织炎症反应和肺病理改变的机制之一.Objective To observe the effects of simvastatin on smoking induced COPD in the lung tissues of rats with IL-17,IL-21 and retinoid-related orphan nuclear receptor gamma t （ROR γ t） expression,to explore the value of simvastatin in the treatment of COPD and its mechanism.Methods Male SD rats were randomly divided into three groups：control group （n =10）,smoking group （n =11） and treatment group （n =10）.The pathological changes of lung tissue were observed in each group.The distribution of IL-17,IL-21 and RORγt protein in lung tissue of rats were detected by immunohistochemical method.The mRNA expression of IL-17,IL-21 and RORγt in rat lung tissue was measured by real-time RT-PCR.Results In the bronchial epithelium and alveolar septa,the expression of ID17,IL-21 and RORγt positive cells were higher in smoking group [（90.00 ± 14.02） cells/mm2,（61.18 ± 9.16） cells/mm2,（77.27 ± 7.95） cells/mm2] and treatment group [（72.80 ± 10.79） cells/mm2,（49.40 ± 3.34） cells/mm2,（65.40 ± 5.23） cells/mm2] compared with control group [（5.20 ± 4.49） cells/mm2,（8.20 ± 3.16） cells/mm2,（6.80 ± 5.85） cells/mm2] （P 〈 0.01）,and the expression of smoking group was higher than the treatment group （P 〈0.05）.The mRNA expression of IL-17,IL-21 and RORγt in lung tissue of smoking group （7.49 ± 2.93,12.22 ± 4.98,4.04 ± 0.21） and treatment group （2.39 ± 0.62,3.78 ± 0.35,1.76 ± 0.25） were higher than those in control group （1.00 ± 0.00,1.00 ± 0.00,1.00 ± 0.00） （P 〈0.01）,and the expression level in smoking group was higher than that in control group （P 〈 0.05）.There was a positive correlation between the number of IL-17 positive cells in the alveolar septum and the mean linear intercept （r =0.729,P 〈0.01）,which was negatively correlated with the mean alveolar number （r =-0.889,P 〈0.01）.Conclusions IL-17,IL-21 and RORγt plays a role in the process of COPD formation in rats,simvastatin reduced expression of inflammatory cells and

关 键 词：慢性阻塞性肺疾病 白介素17 白介素21 转录因子视黄酸相关孤儿受体 yt 辛伐 他汀 

分 类 号：R563.9[医药卫生—呼吸系统]