氯丙嗪上调大鼠脑皮质中凝血因子Ⅲ的表达  

Chlorpromazine up-regulates the expression of coagulation factor Ⅲ in rat cortex

作　　者：吴静[1] 李洋[2] 韩秋媛 于丽[1] 钱钧[1] 张文莉[1] 李爱梅[1] 李玉军[1] 张庆猛[1] 宋武琦[1] 考文萍[1] 潘超[1] 张凤民[1] 

机构地区：[1]哈尔滨医科大学微生物学教研室,伍连德研究所,黑龙江省感染与免疫重点实验室,150081 [2]哈尔滨医科大学附属第三医院放疗科,150081 [3]哈尔滨医科大学附属第二医院重症医学科,150081

出　　处：《国际遗传学杂志》2017年第1期13-18,共6页International Journal of Genetics

基　　金：基金项目：黑龙江省教育厅科技研究项目（12541492）;国家自然科学基金青年科学基金项目（81601428）

摘　　要：目的对氯丙嗪（chlorpromazine，CPZ）处理后的大鼠脑皮质中凝血因子Ⅲ表达进行检测及分析。方法按每组6只，将wistar大鼠随机分为：正常对照组、CPZ高、中、低剂量（20mg／kg、10mg／kg、5mg／kg）单次注射组以及中剂量连续注射组（10mg／kg／2周），采用腹腔注射CPZ的方法制备动物模型。免疫组化和Western印迹法检测大鼠脑皮质中凝血因子Ⅲ的表达。结果免疫组化结果显示，中剂量（10rag／kg）的CPZ单次注射及连续注射均能显著增加大鼠啮皮质中凝血因子Ⅲ的表达（P值分别为0．0007、0．0005）。Western印迹结果显示，与正常对照组相比，CPZ高、中剂量（20mg／kg、10mg／kg）单次注射（P值分别为0．0141、0．0163）以及中剂量（10mg／kg）长期连续注射组大鼠脑皮质中凝血因子Ⅲ表达显著增加（P=0．0094）。结论CPZ能够上调大鼠脑皮质中凝血因子Ⅲ的表达。除了已知的多巴胺受体和钙调素之外，凝血因子Ⅲ也是CPZ调控的靶分子之一。Objective To analyze the expression of coagulation factor Ⅲ in chlorpromazine （ CPZ ） - treated rat cortex. Methods Wistar rats （ n = 6 ） were assigned to normal control and CPZ trea- ted groups. The rats of CPZ treated group were injected intraperitoneally with CPZ （5 mg/kg, 10 mg/kg and 20 rag/kg for single-injection or once-daily injection （ 10 mg/kg ） continuously for 2 weeks. Immu- nohistochemistry and Western blot analysis were performed to examine the expression level of coagulation factor Ⅲ in rat cortex. Results Immunohistochemistry results showed that both the single and continuous repeated use of CPZ （ 10 mg/kg ） could lead to a significant increase in coagulation factor Ⅲ expression in rat cortex compared to control subjects （ P = 0. 0007, 0. 0005 ）. Quantification of the Western blot indicates that both the 10 mg/kg and 20 mg/kg dose of CPZ single injection significantly increased the coagulation factor Ⅲ expression level in rat cortex （ P = 0.0141 , 0. 0163 ）. Similarly, continuous treatment of CPZ （ 10 mg/kg ） also resulted in a significant increase of coagulation factor m in rat cortex compared with the untreated control group （P = 0. 009 ）. Conclusion CPZ could up-regulate the coagu- lation factor Ⅲ expression in rat cortex. Except for the dopamine receptor and calmodulince, coagulation factor Ⅲ is also a target protein of CPZ.

关 键 词：氯丙嗪 大鼠 脑皮质 凝血因子Ⅲ 

分 类 号：R965[医药卫生—药理学]