荧光光度法测定银铈氧化锌纳米复合材料中自由基含量  

Determination of The Free Radical Content in The Nano-Silver Cerium-Zinc Oxide by Fluorescence Spectrophotometry

作　　者：于连生[1] 李秀玲[1] 叶彩[1] 佟德成[1] 秦宇珊 郭强[1] 宋笛[1] 吕玉光[1] 

机构地区：[1]佳木斯大学药学院,黑龙江佳木斯市154007

出　　处：《现代科学仪器》2017年第1期85-87,共3页Modern Scientific Instruments

基　　金：国家自然科学基金项目（（No.21346006））,佳木斯大学科研面上项目（JMSUJCMS2016-013）和佳木斯大学研究生科技创新项目YM2016_093）.

摘　　要：采用成熟制备方法制备出平均粒径大约为50nm左右的Ag-Ce-ZnO纳米复合材料，为了解决其团聚的问题，实验中采用表面活性剂聚丙烯酸钠（PAAS）处理纳米复合材料。经表面活性剂处理后的纳米复合材料平均粒径大约为30nm左右，应用荧光光度法测定表面活性剂PAAS对纳米复合材料产生羟基自由基含量的影响。在测定羟自由基的新体系时利用其产生的羟基自由基氧化水杨基荧光酮，使水杨基荧光酮的荧光强度发生变化，从而间接地测定羟自由基的产生量。实验结果表明，表面活性剂PAAS的加入能够有效的减小纳米复合材料的平均粒径，增加其产生的羟基自由基含量，使纳米复合材料得到良好的分散。Using a practical preparation method, the average particle size of manufactured Ag-Ce-ZnO nanoscale composite materials is about 50nm. In order to solve the problem of the aggregation, it is treated by surfactant sodium polyacrylate （PAAS） nanoscale composites. After surfactant treatment, the average particle size of nanometer composite material is about 30nm. It is measured that surfactant PAAS hydroxyl radicals content influence on nanometer composites by the fluorescence spectrophotometry. To determination hydroxyl radicals in the new system, the hydroxyl radicals generated by oxidation of salicylfluorone is used. According as the fluorescence intensity of salicylfluorone variation, the amount of hydroxyl radical production can be measured indirectly. The experimental results showed that the addition of surfactant PAAS can reduce effectively the average particle size of nanometer composite material, increase the content of hydroxyl radicals, and make the nanometer composite.materials lead to good dispersion.

关 键 词：纳米复合材料 聚丙烯酸钠 荧光光度法 水杨基荧光酮 

分 类 号：TB33[一般工业技术—材料科学与工程]