对羟基苯甲酸丁酯通过氧化应激导致人精子活力降低和凋亡增加  

Increase of apoptosis and decrease of sperm motility induced by oxidative stress after exposed to butyl p-hydroxybenzoate

作　　者：李东航[1] 袁冬[1,2] 张林媛[1] 乔佩环[1] 梁晓薇[1,2] 常兵[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050 [2]国家卫生计生委男性生殖健康重点实验室,国家人口计生委科学技术研究所

出　　处：《卫生研究》2017年第2期196-200,共5页Journal of Hygiene Research

基　　金：国家自然科学基金(No.30972447)

摘　　要：目的探究对羟基苯甲酸丁酯(BP)对人精子活力、氧化应激和细胞凋亡的影响及其机制。方法选取人精液样本20份,随机分为4组:对照组及BP低、中、高浓度组,每组5个平行样。与BP体外共培养4 h,BP染毒浓度分别为0、200、400和800μmol/L。观察精子活动力、细胞毒性、活性氧阳性细胞率和细胞凋亡率。结果BP低、中、高浓度组精子总活力[(26.44±7.83)%、(32.79±2.90)%和(10.51±5.88)%]均低于对照组[(47.67±3.93)%],差异有统计学意义(P<0.05)。与对照组相比,BP低、中、高浓度组精子存活率分别为(63.36±9.08)%、(49.72±7.15)%和(29.91±5.93)%。BP低、中、高浓度组活性氧阳性细胞率[(24.67±0.50)%、(54.50±3.40)%和(59.93±3.47)%]高于对照组[(8.63±0.57)%],差异有统计学意义(P<0.05),晚期精子凋亡率[(11.8±1.74)%、(12.87±0.25)%和(14.60±0.87)%]高于对照组[(9.63±1.00)%],差异有统计学意义(P<0.05)。精子活性氧的水平和晚期凋亡率均与精子的总活力呈负相关,r分别为-0.727和-0.688(P<0.05)。结论对羟基苯甲酸丁酯具有细胞毒性,能够降低精子活动能力,促进精子活性氧的生成和细胞凋亡。Objective To explore the effect and mechanism of butyl phydroxybenzoate（ BP） on sperm motility,oxidative stress and apoptosis. Methods Semen samples from 20 healthy sperm donors were randomly divided into four groups：group control,BP 200 μmol/L,BP 400 μmol/L and BP 800 μmol/L,each group had five parallel samples. The spermatozoa were cultured with BP for 4 h in vitro,with the exposed concentration of BP at 0,200,400 and 800 μmol/L,respectively. The influence of BP on spermatozoa were analyzed by sperm activity,cytotoxicity,the rate of reactive oxygen species（ ROS） positive cell and apoptosis. Results The total sperm activity in group BP200 μmol/L,BP 400 μmol/L and BP 800 μmol/L were（ 47. 67 ± 3. 93） %,（ 32. 79 ±2. 90） %,（ 10. 51 ± 5. 88） % respectively,which were significantly lower than control（ 26. 44 ± 7. 83） %. Compared with the control group,the survival rate of sperm in the three experiment groups were（ 63. 36 ± 9. 08） %,（ 49. 72 ± 7. 15） %,（ 29. 91 ±5. 93） % respectively. Rate of ROS positive cells in the three experiment groups were（ 24. 67 ± 0. 50） %,（ 54. 50 ± 3. 40） %,（ 59. 93 ± 3. 47） % respectively,which were significantly higher than control（ 8. 63 ± 0. 57） %. The rate of late stage apoptosis were（ 11. 8 ± 1. 74） %,（ 12. 87 ± 0. 25） %,（ 14. 60 ± 0. 87） % respectively,which were significantly higher than control（ 9. 63 ± 1. 00） %. The level of sperm ROS and the late stage apoptosis rate were negatively correlated with the total sperm motility,correlation coefficient were- 0. 727 and- 0. 688 respectively（ P 0. 05）. Conclusion BP has cytotoxicity and can reduce sperm motility and promote the production of sperm ROS.

关 键 词：对羟基苯甲酸丁酯 精子 细胞毒性 活性氧 凋亡 

分 类 号：R994.6[医药卫生—毒理学] Q132.7[医药卫生—药学]