北美海棠组培脱菌技术的优化  

Optimization of Technology for North American Begonia Taking off Bacteria by Tissue Culture

作　　者：黄俊轩[1] 刘艳军[1] 杨静慧[1] 李建科[1] 李雕[1] 

机构地区：[1]天津农学院园艺园林学院,天津300384

出　　处：《北方园艺》2017年第6期113-116,共4页Northern Horticulture

基　　金：天津市科委资助项目(14ZXNZNC0040)

摘　　要：以北美海棠消毒处理的一年生枝腋芽为试材,采用组织培养技术与抗生素结合的方法,研究了不同抗生素种类与浓度以及不同的处理时间对试材脱菌效果的影响。结果表明:北美海棠外植体在含有羧苄青霉素(250mg·L^(-1))+卡那霉素(50mg·L^(-1))的MS培养基中获得的脱菌苗生长效果最好;经过在增殖培养基上的增殖筛选和3次继代培养,获得的再生苗生长势稳定率最高;经过对再生脱菌苗的细菌学检测,基本没有微生物出现;该试验结果可以为北美海棠的优质苗木繁殖提供技术支撑。The North American Begoniaannual branches axillary buds with disinfection treatment were used as test materials.By the method of tissue culture technology combined with antibiotic,different kinds of antibiotics and concentration was studied in order to find the effection of taking off the bacteria.The results showed that on the surface of MS medium containing carboxy benzyl penicillin（250mg·L^-1）＋kanamycin（50mg·L^-1）,the effection of taking off the bacteria was the best;after screened on the multiplication culture medium and three times successive transfer culture,the stability factor of growth potential for regeneration seedling was the highest.After the bacteriological tests of the vaccine for the regeneration seedlings,microbes could not be found on the culture medium.The test results would be the technical support for North American Begonia quality nursery stock breeding.

关 键 词：北美海棠 组织培养 抗生素 脱菌 

分 类 号：S687.603.6[农业科学—观赏园艺]