一株产聚-β-羟基丁酸酯(PHB)的嗜盐菌Halomonas sp.发酵条件的优化及产物特性鉴定  

Fermentation condition and product identification on the PHB accumulating Halomonas strain

作　　者：刘润泽[1] 王海飙[1] 常乐[1] 刘长莉[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《黑龙江大学自然科学学报》2017年第1期89-94,共6页Journal of Natural Science of Heilongjiang University

基　　金：黑龙江省自然科学基金资助项目(LC2016005);东北林业大学大学生科研训练资助项目(KY2015051)

摘　　要：对一株合成聚-β-羟基脂肪酸酯(PHB)的Halomonas DQ-4发酵过程中碳、碳氮比、温度、pH值、转速及装液量等条件因子进行了优化。结果表明,最佳发酵条件为:碳源为葡萄糖,碳氮比为100/7,生长温度38℃,pH值为9,转速为160 r·min^(-1),装液量为100 m L/500 m L,连续培养60 h时,PHB产量达到峰值,为5.88 g·L^(-1)。同时,对发酵合成的PHB进行了核磁共振氢谱、红外光谱的表征,热重分析表明,PHB具有良好的热稳定性,并且在降解实验中表现了优良的生物降解性。The fermentation conditions for Halomonas DQ - 4 accumulate poly-β-hydroxybutyrate （ PHB ） such as carbon source, carbon nitrogen ratio, temperature, pH and fluid volume were investigated. These results showed that the optimized conditions were： glucose for carbon source, carbon and nitrogen ratio 100/7, temperature 38℃, pH 9, shaker speed 160 r · min^-1 and fluid volume ratio 100 mL/500 mL. The PHB yield reached up to 5.88 g · L^-1 when it was cultured about 60 h. GCMS analysis confirmed that the product was PHB. Hydrogen nuclear magnetic resonance （NMR）, infrared spectroscopy and thermogravimetric analyses showed this material had a good thermodynamic performance. In addition, PHB degradation experiments showed this material has a good biodegradability .

关 键 词：HALOMONAS 聚-β-羟基脂肪酸酯 最适条件 产物鉴定 

分 类 号：TM25[一般工业技术—材料科学与工程]