RP-HPLC-DAD同时测定复方石韦胶囊中4个黄酮成分的含量  

Simultaneous determination of four flavonoids in Fufang Shiwei capsules by RP-HPLC-DAD

作　　者：杨志欣[1] 王祺茹 李霞[1] 单柏松 刘佳佳[1] 王海威[1] 王艳宏[1] 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《药物分析杂志》2017年第3期427-431,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：黑龙江省自然科学基金(H2016057);国家教育部春晖计划(Z2008-1-15016);黑龙江省教育厅骨干教师基金(No.1251 G058);黑龙江中医药大学"优秀创新人才支持计划"项目(2012)

摘　　要：目的:建立RP-HPLC-DAD方法,同时测定复方石韦胶囊中苦参酮、槐属二氢黄酮G、异黄腐醇、三叶豆紫檀苷4个黄酮含量。方法:采用C_(18)色谱柱(250 mm×4.6 mm,5μm),以甲醇-水为流动相梯度洗脱,流速1mL·min^(-1),检测波长300 nm。结果:苦参酮、槐属二氢黄酮G、异黄腐醇、三叶豆紫檀苷线性范围分别为0.34~6.84μg(r=0.999 3)、0.16~3.27μg(r=0.999 5)、0.04~0.85μg(r=0.999 1)、0.19~3.70μg(r=0.999 8);精密度、重复性良好,RSD均小于2.0%;在室温条件下供试溶液12 h内稳定;平均加样回收率(n=6)在99.2%~101.5%(RSD≤2.2%)。3批样品中上述4个成分平均含量依次为94.84~98.85、44.73~47.79、11.16~13.26、54.17~58.74μg·粒^(-1)。结论:所建立的分析方法可用于复方石韦胶囊中4个黄酮成分的含量测定。Objective：To establish a method for simultaneous determination of four flavonoids in Fufang Shiwei capsules.Methods：Analysis was carried out on a Diamonsil C_（18） column（250 mm×4.6 mm,5 μm）using methanol-water as mobile phase at the flow rate of 1mL·min（-1） for gradient elution and the detection wavelength was 300 nm.Results：The calibration curves of the four analytes exhibited good linearity over the range of 0.34-6.84 μg（r =0.999 3）for kurarinone,0.16-3.27 μg（r =0.999 5）for sophoraflavanone G,0.04-0.85 μg（r =0.999 1）for isoxanthohumol,and 0.19-3.70 μg（r =0.999 8）for trifolirhizin.The precision and the repeatability were good with RSD value less than 2.0%.The sample solution was stable in 12 h at room temperature.The average recoveries（n=6）ranged from 99.21% to 101.54%（RSD ≤ 2.19%）.The average contents of 3 batches of samples were 94.84-98.85 μg per capsule for kurarinone,44.73-47.79 μg per capsule for sophoraflavanone G,11.16（-1）3.26 μg per capsule for isoxanthohumol and 54.17-58.74 μg per capsule for trifolirhizin,respectively.Conclusion：The method is suitable for simultaneous determination of four flavonoids in Fufang Shiwei Capsules.

关 键 词：复方石韦胶囊 苦参酮 槐属二氢黄酮G 异黄腐醇 三叶豆紫檀苷 黄酮类成分 高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]