RP-HPLC法测定紫杉醇脂质体的药物包封率  

Determination of entrapment efficiency of liposomal paclitaxel by RP-HPLC

作　　者：游杨杨[1] 索绪斌[1] 岳静静[1] 徐兴[1] 张涵 

机构地区：[1]广东药科大学中药学院,广州510006 [2]广州市茗川生物科技有限公司,广州510006

出　　处：《药物分析杂志》2017年第3期535-539,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(81303288);国家科技创新基金(国科发计[2013]583号);广东省教育厅创新强校特色创新项目(粤教科函[2015]3号)

摘　　要：目的:建立紫杉醇脂质体药物包封率的测定方法。方法:采用葡聚糖凝胶G-50柱色谱法分离紫杉醇脂质体和游离药物,用RP-HPLC法检测紫杉醇的量,计算脂质体中紫杉醇的包封率;色谱条件:采用Platisil-ODS(250 mm×4.6 mm,5μm)色谱柱,以甲醇-水(含0.1%三乙胺和0.67%冰乙酸)(80∶20)为流动相,流速1.0mL·min^(-1),检测波长227 nm,柱温为室温。结果:紫杉醇与辅料及溶剂的色谱峰分离良好,峰形很好;紫杉醇线性范围0.5~50μg·mL^(-1)(r=0.999 3),加样回收率范围为99.66%~100.8%,包封率范围为90.29%~91.65%。结论:本方法能快速检测紫杉醇脂质体中紫杉醇含量及包封率。Objective：This study establishes the determination method of entrapment efficiency of paclitaxel liposome.Methods：The liposomal paclitaxel were separated well from free paclitaxel by the Sephadex G-50 chromatography and was detected with RP-HPLC method.A Platisil-ODS（250 mm×4.6 mm,5 μm）analytical column was used at room temperature,and the mobile phase was composed of methanol-water（containing 0.1% triethylamine and 0.67% glacial acetic acid）（80∶20）at a flow rate of 1.0mL·min（-1）,and the detection wavelength was 227 nm.Results：Paclitaxel peak was well separated with liposome excipients peak and solvent peak with a good peak shape.The linear range of paclitaxel was 0.5-50 μg·mL（-1）（r=0.999 3）,the average recovery was 99.66%（-1）00.84% and the entrapment efficiency of the liposomal paclitaxel was 90.29%-91.65%.Conclusion：The method can quickly detect the encapsulation efficiency of liposomal paclitaxel.

关 键 词：红豆杉 二萜生物碱类化合物 紫杉醇 脂质体 药物载体 包封率测定 葡聚糖凝胶G-50 反相高效液相色谱 

分 类 号：R917[医药卫生—药物分析学]