玄参内生真菌分离、鉴定及系统发育分析  

Identification and phylogenetic analysis of endophytic fungi isolated from Scrophularia ningpoensis

作　　者：伍晓丽[1,2,3,4] 宋旭红[1,2,3,4] 杨宏国[5] 刘飞[4,6] 张雪[1,2,3,4] 刘金亮[1,2,3,4] 陈大霞[1,2,3,4] 李隆云[1,2,3,4] 

机构地区：[1]重庆市中药研究院种植研究所,重庆400065 [2]重庆市中药良种选育与评价工程技术研究中心,重庆400065 [3]重庆市中药资源学重点实验室,重庆400065 [4]中国中医科学院中药资源中心重庆分中心,重庆400065 [5]重庆邮电大学生物信息学院,重庆400065 [6]重庆市中药研究院中心实验室,重庆400065

出　　处：《中国中药杂志》2017年第5期902-911,共10页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2011BAI13B02-2);重庆市科技研发基地项目(cstc2014ptyjd10001);科技富民强县专项行动计划项目(国科发农[2014]160号);重庆市基础与前沿研究计划项目(cstc2013jcyj A10126)

摘　　要：分别从武隆产区玄参根、主茎、侧枝、叶片和重庆市区栽培玄参侧枝中分离内生真菌,根据形态学特征和26Sr DNA D1/D2测序结果对其进行鉴定,并进行了种群多样性分析,对优势属炭疽菌属内生真菌构建了系统发育树。试验结果显示:真菌种群多样性指数,武隆玄参叶片>主茎=侧枝>根,重庆玄参侧枝>武隆玄参侧枝。共计分离得到内生真菌58株,绝大部分是各种植物病原菌,其优势属为刺盘孢属Colletotrichum,其中绝大多数是C.gloeosporioides和C.boninense。叶片和子芽可能是内生菌侵染的主要途径;内生菌和病原菌可能相互转化,受环境、基因型等多种因素影响。The endophytic fungi from root, main stem, branch and leaf of Scrophularia ningpoensis were isolated and identified from Wulong and Chongqing, and the population diversity analysis and phylogenetic analysis were followed. The result indicated that, as to population diversity index, S. ningpoensis from Wulong： leaf 〉 main stem = branch 〉 root, branch from Chongqing 〉 branch from Wulong. Fifty-eight endophytic fungi were obtained, most of which were the pathogens of the plant. Colletotrichum was the prevailing ge-nus, of which C. gloeosporioides and C. boninense were the prevailing strains. Leaf and seedlings might be the main path of infection. Endophytic fungi and pathogen might convert to each other, influenced by such factors as environment, genotype et al.

关 键 词：玄参 内生真菌 鉴定 系统发育分析 

分 类 号：S567.53[农业科学—中草药栽培] Q93[农业科学—作物学]