高效液相法同时测定银杏叶中11种成分的含量  

Simultaneous determination of eleven components in Ginkgo biloba leaves by high performance liquid chromatography method

作　　者：吕金丽[1,2] 杨彪[1] 李孟璇[1] 孟兆青[1] 马世平[2] 王振中[1] 丁岗[1] 黄文哲[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中国药科大学,江苏南京210009

出　　处：《中国中药杂志》2017年第5期931-935,共5页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2013ZX09402203)

摘　　要：为了考察不同产地银杏叶药材中黄酮醇苷和双黄酮的含量差异,本实验采用Agilent ZORBAX SB-C18(4.6 mm×250mm,5μm)色谱柱,在流动相为乙腈-0.4%磷酸水溶液系统,流速1 m L·min-1,检测波长为254 nm的条件下进行梯度洗脱,建立了同时测定银杏叶中7种黄酮醇苷和4种双黄酮成分含量的高效液相色谱方法。测定结果表明10个不同产地的银杏叶药材中7种黄酮醇苷和4种双黄酮成分的含量存在差异。To study Ginkgo biloba leaves in different producing area, we establish an HPLC method for the simultaneously determi- nation of seven flavonoids glycosides and four biflavonoids in G. biloba leaves. The analysis was performed on an Agilent ZORBAX SB- C18 column（4. 6 mm ×250 mm, 5 μm） wich acetonitrile, and 0. 4% phosphoric acid as mobile phase at flow rate of 1 mL · min^-1 in a gradient edution, and the detection was carried out at 254 nm. The calibration curves of the seven flavonoids glycosides and four bifiavonoids had a good linearitiy with good recoveries. The established HPLC method is simple, rapid, accurate, reliable, and sensitive, and can be applied to the identification and quality control of G. biloba leaves.

关 键 词：银杏叶 黄酮醇苷 双黄酮 高效液相法 多成分定量 

分 类 号：R284.1[医药卫生—中药学]