铜绿假单胞菌在不同老年呼吸道感染中的生物学特性研究  

Biologic characteristics of Pseudomonas aeruginosa isolated from elderly with different respiratory infections

作　　者：赵铁梅[1] 杨晓丹[1] 苏宪灵[1] 

机构地区：[1]解放军总医院呼吸内科,北京100853

出　　处：《中华老年多器官疾病杂志》2017年第3期182-185,共4页Chinese Journal of Multiple Organ Diseases in the Elderly

基　　金：解放军总医院临床科研扶持基金(2013FC-TSYS-2001)~~

摘　　要：目的研究慢性阻塞性肺疾病(COPD)急性发作和机械通气相关肺炎(VAP)老年患者呼吸道标本分离所得铜绿假单胞菌的生物学特性是否存在差异。方法收集2012年1月至2014年3月解放军总医院呼吸内科收治的COPD急性发作和VAP患者呼吸道标本分离得到的铜绿假单胞菌共48株,分为两组:COPD组(n=22)和VAP组(n=26)。应用结晶紫染色法检测铜绿假单胞菌的生物被膜形成能力;应用氯仿萃取法测定铜绿假单胞菌的绿脓菌素含量;应用弹性蛋白-刚果红染色方法检测弹性蛋白酶活性。结果 COPD组和VAP组分离所得铜绿假单胞菌的生物被膜形成能力无显著性差异(P>0.05),但存在个体差异。COPD组与VAP组间绿脓菌素和弹性蛋白酶的含量差异无统计学意义(P>0.05),但存在个体差异。结论在COPD急性发作和VAP中,铜绿假单胞菌感染后的生物被膜形成能力和产生的主要毒力因子能力相似。Objective To determine whether there are biologic differences in the Pseudomonas aeruginosa strains isolated from respiratory specimens among the elderly patients with acute exacerbation of chronic obstructive pulmonary disease （ COPD） or those with ventilator associated pneumonia （ VAP） .Methods A total of 48 strains of Pseudomonas aeruginosa isolated from the respiratory specimens of the elderly with acute exacerbation of COPD and VAP who admitted in our department from January 2012 to March 2014 were collected;and among them , 22 were from the COPD patients and 26 from the VAP patients .Biofilm formation was quantified and classified with crystal violet staining .The production of pyocyanin was measured with chloroform extraction method , and the elastase activity was determined with elastin-congo red staining .Results Biofilm formation abilities were similar in the strains isolated from respiratory specimens of COPD group and VAP group （P〉0.05）, but there existed individual differences .No obvious differences were observed in the production of main virulence factors （pyocyanin and elastase） between the 2 groups （P〉0.05）.Conclusion Pseudomonas aeruginosa strains from the elderly with acute exacerbation of COPD and VAP have similar biofilm formation abilities and production of main virulence factors .

关 键 词：铜绿假单胞菌 慢性阻塞性肺疾病 机械通气相关肺炎 生物被膜 毒力因子 

分 类 号：R563.9[医药卫生—呼吸系统] R592[医药卫生—内科学]