细胞学方法评价6种中药的寒热药性  

Evaluation on Heat and Cold Characteristics of Six TCM Herbs by Cytological Method

作　　者：王亚男[1] 窦德强[1] 

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《辽宁中医杂志》2017年第3期558-560,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)(2013CB531803)

摘　　要：目的:评价6种中药的寒热属性。方法:用噻唑蓝(MTT)比色法考察黄芩、黄连、黄柏、干姜、附子、花椒对人正常肝细胞LO2以及人肺癌细胞A549细胞体外增殖的影响,并用倒置显微镜观察黄芩、黄连、黄柏、干姜、附子、花椒对细胞形态特征的影响,台盼蓝染色法观察黄芩、黄连、黄柏、干姜、附子、花椒对LO2以及A549细胞的毒性作用。结果:黄芩、黄连、黄柏在所选浓度范围内抑制两种细胞生长,使细胞固缩,变圆;干姜、附子、花椒在低浓度时促进两种细胞生长,使细胞密度增加、结构致密。台盼蓝染色表明6种中药对两种细胞均无细胞毒性。结论:黄芩、黄连、黄柏药性为寒凉,干姜、附子、花椒药性为温热;黄芩、黄连、黄柏、干姜、附子、花椒对LO2及A549细胞均无细胞毒活性。Objective： To evaluate the heat and cold characteristics of six traditional Chinese medicine（ TCM） herbs. Methods：MTT assay was utilized to observe the effect of scutellariae radix,Chinese goldthread,phellodendron amurense rupr,dry ginger,mankshood,pericarpium zanthoxyli on the proliferation of LO2 cells and A549 cells in vitro. Morphological changes were observed by using the inverted microscope. Analyze the cytotoxicity of LO2 cells and A549 cells tested by Trypan-blue staining. Results：Scutellariae radix,Chinese goldthread,phellodendron amurense rupr could inhibit the proliferation of LO2 cells and A549 cells in the selected concentration range. The morphological observation showed three TCM herbs could decrease the cell density and make cells turn round. Dry ginger,mankshood,pericarpium zanthoxyli could promote the proliferation of LO2 cells and A549 cells in the low concentration range. The morphological observation showed it could increase the cell density and structure density. Trypan-blue staining showed six herbs had no cytotoxicity. Conclusion： Scutellariae radix,Chinese goldthread,phellodendron amurense rupr are cold drugs,dry ginger,mankshood,pericarpium zanthoxyli are hot drugs,and six kinds of TCM herbs had no cytotoxicity.

关 键 词：寒热药性 MTT法 LO2细胞 A549细胞 

分 类 号：R284[医药卫生—中药学]