益气解毒活络中药有效成分对高糖刺激人肾小球系膜细胞增殖分化及ColIV、MCP-1 mRNA和蛋白表达影响  

Effect of Active Components of Supplementing Qi,Detoxification Activating Blood Circulation and Dredging Collaterals Chinese Herbs on Proliferation and Differentiation and mRNA and Protein Expressions of Col IV,MCP-1 of HMC Induced by High Glucose

作　　者：辛彩虹[1] 李敬林[2] 郑洪新[1] 苏麒麟 林庶如[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁中医药大学附属医院,辽宁沈阳110032

出　　处：《辽宁中医杂志》2017年第3期566-570,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81173153)

摘　　要：目的:观察高糖刺激下人肾小球系膜细增殖分化及Col IV、MCP-1 mRNA和蛋白活性表达变化,探究益气解毒活络中药有效成分防治糖尿病肾病的分子机制,讨论正交配伍中最优药物组合。方法:以体外培养人肾小球系膜细胞株,传代3~5代后接种于96孔培养板,采用4因素3水平分为正常对照组,高糖模型组、中药1、2、3、4、5、6、7、8、9组,以相应药物对HMCs干预24、48、72 h。MTT法检测24、48、72 h各时间点HMCs增殖分化情况;以qRT-PCR法检测48 h Col IV、MCP-1 mRNA表达;以ELISA法检测48 h Col IV、MCP-1蛋白活性表达。结果:(1)高糖环境刺激作用下HMCs及胞外基质均能够显著增殖,且在48 h达到顶峰;(2)益气解毒活络中药有效成分药防治DN作用机制与通过下调高糖作用下HMCs Col IV、MCP-1 mRNA及蛋白活性表达,进而抑制高糖刺激HMCs增殖相关;(3)益气解毒活络中药有效,最佳配伍为黄芪甲苷低剂量、盐酸小檗碱低剂量、水蛭素低剂量、木犀草苷低剂量。Objective： To observe the mRNA and protein expressions of Col IV and MCP-1 in HMC with high glucose and fat-induced and discuss the prevention and treatment of diabetic nephorpathy and discuss the best scheme of orthogonal compatibility. Methods： HMCin vitro cultured were divided into 11 groups common control group model control group and 9 orthogonal groups. MTT method was used to quantitatively detect the proliferation and differentiation of HMC. The qRT-PCR method was used for detection of expressions of Col IV,MCP-1 mRNA and ELISA method was used for test the expression activity and content of Col IV,MCP-1. Results：（ 1） HMC in vitro was highly proliferation anddifferentiation.（ 2） Active components of supplementing qi and detoxification activating can down regulate the protein and mRNA expressions of Col IV and MCP-1 in HMC with high glucose and fat-induced.（ 3） The best scheme of orthogonal compatibility is low dose of astragaloside IV,low dose of berberine,low dose of Hirudin and low dose of Cynaroside. Conclusion：

关 键 词：中医中药 糖尿病肾病 肾小球系膜细胞 Col IV MCP-1 

分 类 号：R285.5[医药卫生—中药学]