温郁金水提物对Aβ_(25-35)致阿尔兹海默病小鼠模型行为学的影响  

Study of Curcuma Aromatica Extract on Learning and Memory Function in Mice Induced by β-amyloid(Aβ_(25-35))

作　　者：李纪彤[1] 齐越[2] 姜鸿[2] 贾冬[3] 康凯[3] 张冰冰[3] 黄寓[4] 

机构地区：[1]辽宁中医药大学附属三院,辽宁沈阳110034 [2]辽宁中医药大学附属二院,辽宁沈阳110034 [3]辽宁中医药大学,辽宁沈阳110847 [4]沈阳市第二中医医院,辽宁沈阳110111

出　　处：《辽宁中医杂志》2017年第3期633-636,I0006,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家十二五重大新药创制专项课题(2012X09102201-005)

摘　　要：目的:研究温郁金水提物对β-淀粉样蛋白(Aβ_(25-35))致阿尔茨海默病模型(AD)小鼠的学习记忆改善作用。方法:60只昆明种鼠随机分为假手术组,模型组,郁金水提物低(8.35 g生药·g提取物-1)、高(25.05 g生药·g提取物-1)剂量组,盐酸多奈哌齐组(1.30 mg·kg^(-1))及参枝苓组(2.6 m L·kg^(-1)),海马内注射(Aβ_(25-35)),制作小鼠AD模型。连续灌胃给药15 d。Y迷宫和Morris水迷宫实验检测各组小鼠认知行为改变,HE染色观察其对AD模型小鼠神经元的影响。结果:温郁金水提物可显著增加AD模型小鼠的自发交替反应率,缩短逃避潜伏期,减少总路程,延长游泳路程百分比;可改善海马区神经元细胞排列紊乱、水肿等现象,减少神经元死亡。结论:温郁金水提物对Aβ_(25-35)致阿尔茨海默病模型小鼠学习记忆能力具有改善作用。Objective： To investigate the effects of curcuma aromatica extract on learning and memory in mice induced by β-amyloid（（Aβ25-35）. Methods： Sixty mice were divided into six groups randomly： sham group,model group,curcuma aromatica extract low dose group（ 8. 35 crude drug·g extract^-1）,high dose group（ 9. 36 crude drug·g extract-1）,donepezil group（ 1. 30mg·kg^-1） and Shenzhiling group（ 2. 6 m L·kg^-1）. β-Amyloid protein（ Aβ25-35） was infused into the hippocampal of mice.Drugs were administrated for 15 days. Y maze and Morris water maze were used to detect to evaluate the cognitive behavior. HE-staining was used to observe the neuron changes caused by curcuma aromatica extract. Results： Curcuma aromatica extract can increase the alternation behavior significantly,decrease swimming escape latency and total distance,prolong the swimming distance percentage. It also can improve the disorder,edema and other symptoms of hippocampus neurons and reduce the death of neurons.Conclusion： Curcuma aromatica extract can improve the effects of learning and memory in mice induced by β-amyloid（ Aβ25-35）.

关 键 词：温郁金水提物 阿尔茨海默病 Β-淀粉样蛋白 认知能力 

分 类 号：R285.5[医药卫生—中药学]