猪肌肉脂肪酸成分的气相色谱测定方法  

Method for determination of fatty acid composition of porcine muscle using gas chromatography

作　　者：王彦平[1,2] 郭建凤[1,2] 呼红梅[1,2] 王诚[1,2] 武英[1,2] 王继英[1,2] 

机构地区：[1]山东省农业科学院畜牧兽医研究所,山东济南250100 [2]山东省畜禽疫病防治与繁育重点实验室,山东济南250100

出　　处：《畜牧与兽医》2017年第3期57-61,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31372293);国家生猪产业技术体系(CARS-36-09B);山东省现代农业产业技术体系生猪创新团队建设项目(SDAIT-08-03)

摘　　要：传统的脂肪酸测定方法步骤繁琐、耗时长、需用试剂种类多且毒性较大。本研究在2008年颁布的国家标准《动植物油脂脂肪酸甲酯制备》中酯交换法的基础上,对肌肉中脂肪酸甲酯化置备方法进行了适量改进,并通过探讨不同氢氧化钾甲醇添加量和酯化时间对脂肪酸测定结果的影响,建立了利用气相色谱法快速测定猪肌肉中脂肪酸的方法。本方法能准确地分离和测定猪背最长肌的10种脂肪酸,其中7种含量较多,占到所测脂肪酸总量的99.71%。该方法具有较好的精密度、重复性和可靠性,是测定猪肉中脂肪酸成分较为理想的方法。The conventional method for determination of fatty acid composition of porcine muscle takes long time and needs several kinds of reagents which are toxic to people.In order to improve the conventional method,in this study,based on the ester exchange method of national standards（GB/T 17376-2008） issued in 2008,we modified the method of preparation of fatty acid methyl ester,and tested the effect of different volumes of potassium hydroxide solution in methanol and saponification time,and developed a fast and practicable method for determination of fatty acid composition of porcine muscle by gas chromatography.Using this method,ten fatty acids could be determined,and seven of them accounted for 99.71% of the total fatty acid in the muscle.The method is accurate,repeatable and robust,and is suitable to determine the fatty acid composition of porcine muscle.

关 键 词：肌肉 脂肪酸 气相色谱法 猪 

分 类 号：S828[农业科学—畜牧学]