法氏囊活性肽前体蛋白CBL N端片段的克隆及抗原表位分析  

Cloning and characteristic analysis of N terminal fragment of the precursor protein CBL of the bursal-derived active peptide

作　　者：宗嫚嫚 梁静泊 谢金峰[1] 周川杰[1] 周广防 冯秀丽[1] 陈溥言[1] 杨梅[2] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]南京农业大学校医院,江苏南京210095

出　　处：《畜牧与兽医》2017年第3期99-103,共5页Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31302067);江苏省自然科学基金(BK20130682);南京农业大学基本科研业务费专项自主创新重点研究项目(KYZ201634)

摘　　要：Casitas B-lineage lymphoma(CBL)是一种广泛存在于法氏囊组织的细胞内蛋白。为探究禽源CBL基因结构及特征,本研究以Gen Bank公布的CBL基因序列为参考,设计合成引物,扩增CBL基因N端片段420 bp。同源性分析发现,CBL-N基因未发生明显变异,且CBL蛋白具有高度的保守性。Protean软件分析发现,CBL-N蛋白的二级结构比较复杂,含有较多的α和β结构,而且含有一定比例的无规则卷曲及β转角结构,多个区段有较高比例的抗原指数、表面可及性和亲水性,并推测CBL-N中119-136区域氨基酸序列可能是B细胞优势表位。本研究为探讨CBL蛋白功能奠定了基础。Casitas B-lineage lymphoma（CBL） isa widespread intracellular protein in Bursa of Fabricius.In order to explore the structure and characteristics of CBL gene from chicken,according to the CBLsequence published in Gen Bank,a pair of specific primerswere designed and synthesized,and N terminal fragment of CBLgene with 420 bp was amplified.The results of homology analysis showed that CBL gene was highly conservative with no significant variation in nucleic acid sequence and amino acid composition.The results of protean software analysis showed that the secondary structure of CBL-N was complex,including various alpha regions,beta regions and irregular coil regions.Also,the regions with high proportion ofantigen index,surface accessibility and hydrophilicitywere analyzed.It is speculated that the dominant epitopes of B cell in CBL-N protein were located at N-terminal119 to 136 regions according to the hydrophilicity plot,surface probabilityplot and antigenic index.This study provides the foundation for the further study on the function of CBL protein.

关 键 词：CBL 基因克隆 同源性分析 二级结构 B细胞抗原表位 

分 类 号：S855[农业科学—临床兽医学]