力竭运动及钝挫伤对大鼠骨骼肌卫星细胞激活及AXIN/GSK-3β含量的影响  

The Effects of Exhaustive Exercise and Contusion on Satellite Cells Activation and AXIN/GSK-3β Contents in Rat Skeletal Muscle

作　　者：潘同斌[1] 叶雷雷[2] 夏素文 朱超[1] 仝昕炜 汪亚如 

机构地区：[1]扬州大学体育学院,江苏扬州225009 [2]南京体育学院训练处,江苏南京210014

出　　处：《成都体育学院学报》2017年第2期101-106,共6页Journal of Chengdu Sport University

基　　金：江苏省高校自然科学研究面上项目"MG53蛋白在骨骼肌运动损伤修复中的作用及分子机制"(15KJD310001)

摘　　要：目的:研究力竭运动及钝挫伤对大鼠骨骼肌卫星细胞激活及AXIN/GSK-3β含量的影响。方法:取7周龄雄性SD大鼠24只,分为4组,每组6只:对照组(Control,C);力竭运动即刻组(Exhaustive,E0);力竭运动后24h组(E24);力竭运动后48h组(E48)。另取18只SD大鼠,分为3组,每组6只:钝挫伤即刻组(contusion,DO);钝挫伤后24h组(D24);钝挫伤后48h组(D48)。各组分别于安静状态、力竭运动后和钝挫伤后不同时间点,宰杀取血,分离血清。并取左侧股四头肌,一分为二,一份立即进行卫星细胞培养实验,另一份样本液氮保存,测定Axin、GSK-3β含量的变化。另取乳鼠3只,亦取股四头肌进行卫星细胞培养。结果:(1)体外培养结果显示:通过分离消化大鼠肌肉组织,可在体外培养获得高纯度具有成肌能力的肌卫星细胞。培养初期,新生鼠1天后即可见到梭形卫星细胞,其它各组的肌卫星细胞增长速度较慢,到第3天、第5天时各组开始大量增殖梭形细胞,且一直保持良好的增殖、传代,第13天仍长势良好,可见部分融合和微管。各组比较可见,安静对照组骨骼肌卫星细胞数量最少,增殖速度最慢;新生鼠细胞数量最高,增殖速度也最快;而钝挫伤组的骨骼肌卫星细胞数量及增殖速度明显高于力竭运动组。(2)ELISA结果显示:与安静对照组相比,力竭运动各组、钝挫伤各组骨骼肌和血清中的GSK-3β、Axin含量均明显下降(P<0.01),但力竭运动各组(E0、E24、E48)之间和钝挫伤各组(D0、D24、D48)之间无显著差异(P>0.05)。结论:(1)力竭运动及钝挫伤可使肌卫星细胞激活、增殖分化,并且钝挫伤组的骨骼肌卫星细胞激活数量明显多于力竭运动组,可能由于钝挫伤的刺激更强所致。(2)力竭运动、钝挫伤可以下调AXIN、GSK-3β的含量,可能在激活Wnt/β-catenin信号通路及骨骼肌损伤修复中发挥重要作用。Objective：To study the effects of exhaustive exercise and contusion on satellite cells activation and AXIN/ GSK-3β contents in ratskeletal muscle. Methods： 24 male SD rats （seven- week- old）, were randomly di- vided into 4 groups with 6 in each group ： control group （ Control, C） ; immediately - after - exhaustive - exer-cise group （Exhaustive, E0） ; post - exhaustive exercise 24h group （E2d） ; post - exhaustive exercise 48h group （FA8）. Another 18 SD rats were taken and randomly divided into 3 groups with 6 in each group： contu- sion immediate group （ contusion, Do） ; post - contusion 24h group （ D24 ） ; post - contusion 48h group （D48 ）. All groups were killed respectively when at resting state and at different time points after exhaustive ex- ercise and contusion respectively and the serum was extracted. The left quadriceps femoris was sampled and divided into two parts： one was used immediately for satellite cell culture experiment, and the other preserved in liquid nitrogen for measurement of AXIN and GSK -3β contents. The quadriceps femoris of another three neo- natal rats were also takenfor satellite cell culture experiment. Results： （ 1 ） The results of culture in vitro showed that, by separating the rat muscle tissue of digestion, satellite cells with myogenic ability and high purity could be obtained in vitro. During the early stages of culture, a small amount of spindle satellite cells could be seen in the newborn rat the next day after culture, but the satellite cells of the other groups were growing slowly. On the third day and the fifth day, the spindle satellite cells of each group began to proliferate in quantity and reached the peaks. Then all the satellite ceils had a good proliferation until 13th day after serial pas- sage, and fusion and micro - tubuleswere observed in some of them. By comparing the results of each group, we concluded that the content of skeletal muscle satellite cell was lowest in control group, but highest in newborn rats. Th

关 键 词：力竭运动 钝挫伤 肌卫星细胞 轴蛋白 糖原合成酶激酶 

分 类 号：R87[医药卫生—运动医学]