姜黄素与甘草次酸联用对肝癌HepG-2细胞增殖的抑制作用  

Inhibition on proliferation of hepatoma HepG-2 cell treated with Curcumin combined with Glycyrrhetinic acid

作　　者：常明向[1] 吴梅梅[1] 李瀚旻[1] 

机构地区：[1]湖北中医药大学附属医院,湖北武汉430061

出　　处：《药物评价研究》2017年第1期42-47,共6页Drug Evaluation Research

基　　金：国家自然科学基金资助项目(81274147)

摘　　要：目的研究姜黄素与甘草次酸单独、联合用药对肝癌细胞HepG-2增殖的抑制作用,探讨姜黄与甘草配伍的合理性和科学性。方法不同浓度姜黄素(10.00、5.00、2.50、1.25μg/m L)、甘草次酸(20.0、10.0、5.0、2.5μg/m L)及两者对应浓度联合应用处理肝癌HepG-2细胞不同时间后(8、16、24 h),采用CCK-8法检测细胞增殖抑制率;姜黄素5μg/m L、甘草次酸10μg/m L及两者联合应用处理HepG-2细胞24 h后,用流式细胞仪检测细胞凋亡及细胞周期变化情况。结果姜黄素、甘草次酸及联合用药对HepG-2细胞增殖均具有抑制作用,呈剂量相关性和时间相关性,且两药联合呈现相加或增强的作用;姜黄素、甘草次酸均能诱导HepG-2细胞凋亡,联合用药后诱导凋亡作用更强;姜黄素、甘草次酸及联合用药对HepG-2细胞均具有G2期阻滞作用。结论联合用药具有比单用姜黄素或甘草次酸更强的抑制HepG-2细胞增殖、诱导细胞凋亡作用,姜黄与甘草配伍具有合理性和科学性。Objective To study the inhibitory effect of Curcumin combined with Glycyrrhetinic acid on proliferation of HepG-2 cell, and probe the reasonability and scientificity of curcuma combined with glycyrrhiza. Methods The CCK-8 method was used to test the proliferation inhibition rate of HepG-2 cells after treated with Curcumin （10.00, 5.00, 2.50, and 1.25 μg/mL）, Glycyrrhetinic acid （20.0, 10.0, 5.0, and 2.5 μg/mL） and Curcumin combined with Glycyrrhetinic acid in corresponding concentration for different time （8, 16, or 24 h）. Flow cytometry was used to detect the cell apoptosis and the cell cycle of HepG-2 cells after treated with Cureumin （5 μg/mL）, Glycyrrhetinic acid （10 μg/mL） and Curcumin combined with Glycyrrhetinic acid in corresponding concentration for 24 h. Results Cureumin, Glycyrrhetinic acid and drug combination obviously inhibited the proliferation of HepG-2 cells in a time- and concentration-dependent manner, the effects of combination group was more stronger and showed additive effect or synergistic effect. The apoptosis rate of HepG-2 cells was significantly increased after treated with three groups of drug, combination group showed additive effect; Curcumin, Glycyrrhetinic acid and the drug combination showed significant G2 arrest. Conclusion Curcumin combined with Glycyrrhetinic acid has positive effect on inhabiting the proliferation and promoting apoptosis of HepG-2 cell. Curcuma combined with glycyrrhiza possess reasonability and scientificity.

关 键 词：姜黄素 甘草次酸 联合用药 抗肿瘤 凋亡 细胞周期 

分 类 号：R965[医药卫生—药理学]