稀少精子体外培养液对人精子活力的研究  

In vitro culture medium for sparse spermatozoa improves human sperm motility

作　　者：刘丹[1] 黄川[1] 徐孔容 胡静 雷林[1] 袁小波[1] 范立青[1,2] 朱文兵[1,2] 

机构地区：[1]中南大学生殖与干细胞工程研究所 [2]中信湘雅生殖与遗传专科医院,湖南长沙410008

出　　处：《中华男科学杂志》2017年第3期231-236,共6页National Journal of Andrology

摘　　要：目的:探讨稀少精子体外培养液(以下提到处简称激活剂)对人精子活力是否有改善作用,以帮助临床医生、实验室及患者更好的选择辅助生殖方式。方法:本研究选取门诊进行精液常规检查的标本178例,其中弱精子症组151例,正常活力精子组27例。每份标本共取200μl,分成均等的两份,分别加入等体积的激活剂(实验组)和F10(1×)(对照组),然后在37℃、5%的CO_2恒温箱中共孵育30 min,观察并记录两组孵育前后精子的浓度、活力(前向运动、非前向运动及不活动精子百分比)和存活率的变化。结果:激活剂作用于弱精子症组后,精子存活率与作用前及对照组相比,无统计学差异(P>0.05);正常活力精子组呈现出相同的结果。激活剂作用于弱精子症组后,精子前向活力、非前向活力提高的幅度分别是:14.02%和4.86%,而存活型不活动精子比例降低的幅度是19.01%,差异均具有显著性意义(P<0.01);正常活力精子组结果与弱精子症组相似。不论是弱精子症组还是正常活力精子组,存活型不活动精子减少的百分比与存活型不活动精子百分比呈正相关,其r值分别为0.260(P<0.01)(相关程度较弱)和0.679(P<0.01)(相关程度较强)。结论:1激活剂不影响精子存活率;2激活剂提高了弱精子症和正常活力精子的活动力;3精液中存活型不活动精子百分比越高,激活剂作用后,其转变为前向、非前向的百分比越大;且正常活力组的这种相关性强于弱精子症组。Objective： To investigate whether in vitro culture medium （IVCM） for sparse spermatozoa can improve human sperm motility for the purpose of helping clinicians, laboratorians and patients choose a better strategy of assisted reproduction. Methods： Semen samples were obtained from 178 males for routine semen examination from March to August 2016, including 151 ca- ses of asthenozoospermia and 27 cases of normal sperm motility. A total of 200 μ1 was collected from each sample and divided into two equal portions and equal volumes of IVCM （ experimental group） and F10 （ 1 × ） （ control group） were added to the two portions, re- spectively, followed by 30-minute incubation at 37~C in an incubator with 5% CO2. Sperm concentration, motility and viability and the percentages of progressively motile, non-progressively motile and immotile sperm were recorded before and after incubation. Results ： After activated with IVCM, neither the samples with asthenozoospermia nor those with normal sperm motility showed any statistically significant difference in sperm viability from the baseline or the control group （P 〉 0.05）. The rates of progressively and non-progres- sively motile sperm from the asthenozoospermia males were increased by 14.02% and 4.86% respectively, while that of immotile sperm decreased by 19.01% in the experimental group （P 〉 0.01 ） , and similar results were observed in the semen samples from the men with normal sperm motility. The percentage of reduced immotile viable sperm was positively correlated with that of iinmotile viable sperm in both the asthenozoospermia patients （r = 0. 260, P 〈0.01 ） and the men with normal sperm motility （r = 0. 679, P 〈 0.01 ）. Conclusion ： IVCM can increase sperm motility without affecting sperm viability in men with either asthenozoospermia or nor- real sperm motility. The larger the proportion of immotile viable sperm, the higher the percentages of progressively and non-progressive- ly motile sperm in the semen after IVCM acti

关 键 词：精子活力 精子运动增强剂 宫腔内人工受精 体外受精 弱精子症 

分 类 号：R698.2[医药卫生—泌尿科学]