固定化漆酶磁性纳米材料的合成及对合成染料的降解  

PREPARATION OF IMMOBILIZED LACCASE MAGNETIC NANOPARTICLES FOR DEGRADATION OF SYNTHETIC DYES

作　　者：刘莉 黄冬群 鹿毅 

机构地区：[1]新疆产品质量监督检验研究院,乌鲁木齐830011 [2]新疆恒正司法鉴定中心,乌鲁木齐830000

出　　处：《环境工程》2017年第3期38-42,共5页Environmental Engineering

基　　金：国家自然科学基金项目(21177013)

摘　　要：介绍了一种温和无损固定化漆酶的方法,并研究了其对合成染料的降解行为。首先采用共沉淀法合成乙二胺四乙酸(EDTA)功能化的磁性纳米粒子,并以Cu2+为桥基,通过螯合作用将漆酶固载于EDTA功能化的磁性纳米粒子表面。通过透射电镜、紫外-可见光谱仪等对磁性固定化漆酶纳米材料进行了表征。固定化漆酶磁性纳米材料尺寸在15 nm左右,具有良好的磁响应能力,漆酶固定化率可达223 mg/g;在最佳降解条件下,其对孔雀石绿和碱性品红均表现出良好的降解能力;同时固定化漆酶具有较高的稳定性和重复使用性,便于回收利用。A mild and non-destructive method was proposed to immobilize laccase on the surface of EDTA functionalized magnetic Fe3O4 nanoparticles synthesized by coprecipitation step through chelating interaction with Cu（ II） as the bridge group. After being characterized by scanning electron microscopy and UV-visible spectroscopy,the as-prepared absorbents,with a mean diameter of 15 nm,showed a strong magnetic response to an externally applied magnetic field. The fixing amount of laccase was up to 223 mg/g. Under optimum conditions,the immobilized lacasse showed high efficiency for degrading model dyes of Malachite Green and Basic Fuchsine. Meanwhile,it also exhibited high stability and reusage,and easy recovery from the reaction system.

关 键 词：固定化漆酶 磁性纳米材料 合成染料 

分 类 号：X703[环境科学与工程—环境工程] TB383.1[一般工业技术—材料科学与工程]