鸭源致病性大肠杆菌的分离鉴定及系统发育分析  

Isolation,identification and phylogenetic analysis of avian pathogenic Escherichia coli from duck

作　　者：孙畅[1,2] 赵丽丽[2] 徐丽晶[3] 牛银杰 尹海畅[2] 陆涛峰[2] 张圆圆[2] 弥春霞[1] 陈洪岩[2] 

机构地区：[1]牡丹江师范学院生命科学与技术学院,黑龙江牡丹江157011 [2]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室,黑龙江哈尔滨150069 [3]东北农业大学生命科学学院,黑龙江哈尔滨150030

出　　处：《中国兽医科学》2017年第3期286-294,共9页Chinese Veterinary Science

基　　金：国家科技支撑计划项目(2015BA I07B02-02);黑龙江省自然科学基金重点项目(ZD2016006)

摘　　要：为确定江苏省徐州市某养鸭场病死鸭的病原菌,从发病鸭的肝中分离到1株细菌并命名为JSE 4,根据形态特征、培养特性、生化试验、16S r R N A分子鉴定结果可确定该菌为大肠杆菌(E.coli)。对该分离菌进行致病型快速鉴定、动物致病试验、毒力基因检测、药敏试验、系统发育分析,结果显示,该分离株为禽致病性大肠杆菌(APEC),可引起试验组SPF雏鸭死亡,死亡率为16.7%。分离株含有14种毒力基因中的12种,29种常用抗菌药物中,该分离株仅对链霉素、呋喃妥因、亚胺培南、呋喃唑酮、头孢他啶、氨曲南这6种药物具有敏感性。该分离株与源自比利时水源、瑞士禽肉源和中国鸭源分离株的亲缘关系最近,与比利时水源、瑞士禽肉源分离株的同源性高达99.7%。研究表明,从江苏徐州周边养鸭场病死鸭中分离到1株多重耐药的禽致病性大肠杆菌。In order to determine the pathogenic bacteria in a duck-farm from Xuzhou City of Jiangsu Province,a strain,named as JSE4,was isolated from the liver of a dead duck,identified as Escherichia coli based on its colony morphology,microscopic characteristics,biochemical test and 16S rRNA sequence.Rapid detection of avian pathogenic E.coli,virulence assay,virulence genes,drug sensitive test and phylogenetic analysis showed that the strain was avian pathogenic E.coli,and it could cause 16.7%death of the experimental group of ducks.The isolate contained 12 of 14 virulence genes.The result of resistance test to 30 drugs showed that the strain was highly sensitive to streptomycin,macrodantin,imipenem,furazolidone,ceftazidime and aztreonam.16 S rRNA sequence analysis showed that the strain had a close genetic distance with strains from Belgium water,Switzerland poultry meat and China duck,the strain had 99.7%homology to isolates from Belgium water and Switzerland poultry meat.The aboveresults indicate that a avian pathogenic E.coli strain with multiple drug resistance（MDR） is isolated from the liver of a dead duck in a duck-farm from Xuzhou City of Jiangsu Province.

关 键 词：禽致病性大肠杆菌 鸭 分离鉴定 药敏试验 系统发育学分析 

分 类 号：S852.612[农业科学—基础兽医学]