鸡柏氏禽刺螨感染的初步研究  

Preliminary studies on Ornithonyssus bacoti infestation in chicken

作　　者：杨泽晓[1] 刘亚东[1] 李俐睿 姚学萍[1] 王印[1] 周丽军[1] 蒙正群 罗怡琳[1] 李岩[3] 

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]成都市动物疫病预防控制中心,四川成都610041 [3]四川农业大学动物科技学院,四川成都611130

出　　处：《中国兽医科学》2017年第3期301-308,共8页Chinese Veterinary Science

基　　金：四川省科技计划项目(2016NZYZF0059);"十二五"国家科技支撑计划项目(2013BAD12B04)

摘　　要：为探索四川某鸡场近年来肉鸡每年夏季均发生以腹部、腿部皮肤出现丘疹与痂皮为特征性病变的疫病病因,根据送检病鸡的临床症状、剖检病变和疾病流行特点进行初步诊断,并采用常规实验室诊断方法和PCR技术进行寄生虫检查、细菌分离鉴定、鉴别诊断和治疗的初步研究。结果显示,从病鸡体表检查到的禽刺螨为柏氏禽刺螨,PCR扩增其线粒体细胞色素氧化酶亚单位1(CO 1)基因的576 bp大小靶片段与柏氏禽刺螨OB株核苷酸序列的同源性为99%;同时从丘疹病变组织中分离到1株阿尼蒂斯葡萄球菌,该葡萄球菌分离株不致死昆明小鼠,耐受青霉素、阿莫西林、环丙沙星、头孢他啶、复方新诺明、卡那霉素和强力霉素等13种受试药物;确定此疫病由鸡柏氏禽刺螨感染引起,灭鼠、杀螨和敏感药物抗菌措施具有良好防治效果。这是国内鸡发生柏氏禽刺螨感染的首次报道,为鸡禽刺螨感染的防控及相关研究提供了科学参考。The aim of the study was to determine the causes of an avian disease with characteristic lesions of papules and scab on the abdominal part and leg skin,which occurred in a poultry farm in summer of every years recently. The disease was primarily diagnosed according to the clinical symptoms,autopsy pathological changes and epidemiological characteristics. Then its preliminary studies including parasite examination, bacteria isolation, differential diagnosis and treatment were carried out by the conventional laboratory diagnostic methods and PCR technology. The results showed the haematophagous mites collected from the sick cocks were identified as Ornithonyssus bacoti（tropical rat mite）,based on preliminary light microscopy and sequence analysis of a 576-bp PCR target fragments of the mitochondrial cytochrome oxidase subunit 1 gene（CO1）,which showed 99% sequence similarity to O. bacoti isolate OB by BLAST analysis.One strain of Staphylococcus agnetis was isolated from the papules lesions, and the S. agnetis strain was not lethal to KM mice and resistant to penicillin,amoxicillin,ciprofloxacin,ceftazidime,paediatric compound sulfamethoxazole,kanamycin and doxycycline etc.The disease was conclusively diagnosed as cock O. bacoti infestation,and the deratization, acaricidal and use of sensitive antibiotics will give good control effects.It is the first report of avian O.bacoti infestation in China,and provides a basis for the studies and the prevention and control of haematophagous mites infestation in poultry.

关 键 词：皮炎 柏氏禽刺螨 葡萄球菌 

分 类 号：S852.746[农业科学—基础兽医学]