PADV3自然感染猪组织中病毒含量的实时荧光定量PCR检测  

Detection of porcine adenovirus type 3 in the naturally infected pigs by real-time quantitative PCR

作　　者：吕雯婷[1] 方和俊[1] 赵军[1] 李萍[1] 许思遥 杨凡[1] 徐志文[1,2] 朱玲[1,2] 

机构地区：[1]四川农业大学动物医学院,四川成都611130 [2]四川农业大学动物疫病与人类健康四川省重点实验室,四川成都611130

出　　处：《中国兽医科学》2017年第3期309-314,共6页Chinese Veterinary Science

基　　金：四川省科技支撑计划项目(2014NZ0043);教育部"长江学者与创新团队发展计划"项目(IRT13083);四川省"十三五"育种攻关项目(2016NYZ0052);国家"十二五"科技支撑计划(2015BAD12B04-2.3)

摘　　要：为了解猪腺病毒3型(PADV3)在自然感染猪体内不同器官组织中的分布情况,利用实时荧光定量PCR检测方法,定量检测了自然感染PADV3的仔猪、妊娠母猪以及与猪圆环病毒2型(PCV2)混合感染的仔猪不同器官组织中PADV3病毒的含量。结果,在感染的猪体多种器官组织中均可检测到PADV3,但是在各器官组织中病毒的含量有差异,其中肠黏膜、肺组织、肠系膜淋巴结、扁桃体中病毒含量较高,心、肝、脾组织中病毒含量较低;在同时混合感染PCV2的仔猪肺组织、肠系膜淋巴结、扁桃体中PADV3含量显著增加。结果表明,PADV3对猪肠黏膜、肺组织、肠系膜淋巴结、扁桃体有较强的嗜性,对心、肝、脾组织的嗜性相对较弱;PCV2混合感染可促进PADV3在仔猪体内的增殖,能增强PADV3对感染器官组织的亲嗜能力。In order to investigate the distribution of PADV3 in the naturally infected pigs, the real- time quantitative PCR was used to detect the virus content of PADV3 in different organs and tissues of piglets,sows and the pigs mixedly infected（PCV2） with natural infection. In result,PADV3 was detected in most organs but the virus content was different in different organs. The content was higher in the intestinal mucosa,lung,lymphoglandulae mesentericae and tonsil,but it was lower in the heart,liver and spleen. The content of PADV3 of the lung,lymphoglandulae mesentericae and tonsil in piglets mixedly infection with of PCV2 was significantly increased. For the intestinal mucosa,lung,lymphoglandulae mesentericae and tonsil,PADV3 was stronger in tropism,but was lower to the heart,liver,spleen. The existence of PCV2 in fection increased the proliferation and replication,and enhanced the pro-infection tissue tropism of PADV3.

关 键 词：猪腺病毒3型 猪圆环病毒2型 实时荧光定量PCR 

分 类 号：S852.659.1[农业科学—基础兽医学]