伪狂犬病病毒和猪圆环病毒2型混合人工感染仔猪排毒的检测  

Detection of dynamic changes of PRV and PCV2 loading and shedding

作　　者：宋春莲[1] 张应国[2] 李永能[1] 尹革芬[1] 李文贵[1] 段博芳[2] 耿孝杰 张雪[1] 凌万旭 颜洪奎 舒相华[1] 

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]云南省动物疫病预防控制中心,云南昆明650200 [3]晋宁县动物检疫和疫病预防控制中心,云南晋宁650600 [4]昆明伟牧得生物科技有限公司,云南昆明650201

出　　处：《中国兽医科学》2017年第3期360-365,共6页Chinese Veterinary Science

基　　金：云南省科技惠民计划项目(2013CH001);昆明市科技合作重点项目(20130201AH023027)

摘　　要：为了解伪狂犬病病毒(PRV)和猪圆环病毒2型(PCV2)感染仔猪后不同时间点的病毒载量及排毒规律,将34头健康仔猪分为4组,第1~3组分别为人工接种PRV、PCV2和PRV+PCV2组,第4组为空白对照组。分别在接种后第3、7、14、21、35天采集试验仔猪的鼻拭子和肛门拭子,用Taq Man荧光定量PCR(q PCR)对其进行定量检测,并分析仔猪排毒的变化规律。结果,接种后第3天,3个试验组仔猪肛门拭子、鼻拭子中均分离到PRV或PCV2;其中PRV单独感染后的病毒载量在第14天达到峰值331.67 copies/L;PCV2单独感染后的病毒载量在第3天达到峰值10 742.01 copies/L;PRV+PCV2混合感染后鼻拭子和肛门拭子的PRV分别在第14天和第7天达峰值980.96 copies/L和2 230.056 copies/L;鼻拭子和肛门拭子PCV2在感染后第14天达到峰值69.413 copies/L和271.72 copies/L;混合感染仔猪于感染后第8~14天发生死亡。3个感染组之间无论是肛门拭子还是鼻拭子,PRV、PCV2病毒载量均差异显著(P<0.05)。上述结果表明,PRV单独感染、PCV2单独感染和二者混合感染均对仔猪造成严重危害,除引起仔猪发病死亡外,这2种病毒均能通过呼吸道和消化道向外排毒。In order to determine the dynamics of pseudorabies virus（PRV）and porcine circovirus type2（PCV2）loading and shedding,34 healthy piglets were divided into 4 groups in this study.The groups 1,2and 3 were inoculated with PRV,PCV2 and PRV＋PCV2,respectively,while group 4 was set as control group.The nasal swab was collected and tested for viruses on the 3rd,7th,14 th,21th and 35 th days after inoculation.The q PCR method was used for quantitative detection of the changes of viruses.In result,PRV and PCV2 were detected from anal swab and nasal swab of the piglets in the 3 experimental groups on3 rd day post-inoculation.In the PRV infection group,the viral load reached the peak of 331.67 copies/L on 14 th day and in the PCV2 infection group,reached a peak value of 10 742.01 copies/L on the 3rd day,after inoculation.PRV reached the peak of 980.96 copies/L and 2 230.056 copies/μL on the 14 th and 7th day and PCV2 reached the peak of 69.413 and 271.72 copies/L on the 14 th day after inoculation.The piglets died from 8th to 14 th day post-inoculation in the PCV2＋PRV co-infection group. The level of PRV and PCV2 in the anal swab and the nasal swab were significantly different among the 3 experimental groups（P〈0.05）. In conclusion, both the PRV or PCV2 single infection and the co-infection seriously harm the piglets,lead the death of piglets and result in shedding the 2 viruses through respiratory tract and digestive tract.

关 键 词：仔猪 伪狂犬病病毒 猪圆环病毒2型 鼻拭子 肛门拭子 排毒规律 

分 类 号：S852.651[农业科学—基础兽医学]