草地早熟禾及其矮化突变材料锈病病原菌鉴定及抗病机制初探  

Rust pathogen identification and mechanism of disease-resistance research on Kentucky bluegrass dwarf mutant

作　　者：甘露[1] 苏浩天 凌欣闻 尹淑霞[1] 

机构地区：[1]北京林业大学草坪研究所

出　　处：《北京林业大学学报》2017年第3期87-92,共6页Journal of Beijing Forestry University

基　　金：国家自然科学基金项目(31302016);深圳市科技计划项目(JSGG20160229155434792)

摘　　要：锈病是草坪草的重要病害,且病原菌种类多,鉴定困难。本研究以草地早熟禾野生型和矮化突变材料中的感病株系为对象,通过观察锈菌的形态特征,并结合ITS和β-tubulin基因序列分析,其中ITS序列的同源比对分析和β-tubulin基因的分子进化分析结果表明,该菌种与小麦禾柄锈菌的同源关系接近,所以初步认定该菌株属于禾柄锈菌,这是国内对禾柄锈菌引起草地早熟禾锈病的首次报道。同时,我们对病原菌诱导后草地早熟禾PR1L、NPR1L基因的表达变化和PRs蛋白表达进行了研究,发现PR1L、NPR1L基因在病菌诱导12 h时的表达量达到了峰值,且在矮化突变植株(A16)中的相对表达量分别达到了8.8-fold、4.5-fold,均大于在对照植株(WT)中的表达量。另外植物的PRs蛋白在禾柄锈菌侵染植物第8天后的表达量明显高于未侵染的植株。初步对草地早熟禾锈病的防御机制进行了探讨,为今后开展草地早熟禾锈病的预防及抗病育种研究奠定基础。Rust is one of the destructive diseases on turfgrass. The species of rust pathogens are multiple and difficult to identify. In order to determine the rust pathogen species on Kentucky bluegrass wild type and dwarf mutant plant,pathogen morphology,along with ITS and β-tubulin gene sequence analysis were carried out. The analysis of ITS by BLAST and phylogenetic analysis of β-tubulin showed that the strains were related to Puccinia graminis of wheat,so we identified it as Puccinia graminis,which is the first report on Kentucky bluegrass in China. Moreover,transcriptional level of PR1L and NPR1L genes in Kentucky bluegrass inoculated with Puccinia graminis,as well as the expression of PRs protein,were investigated. The results presented that the expression of PR1L and NPR1L genes reached peak at 12 hours induced by Puccinia graminis,and the fold changes in dwarf mutant（A16） were 8. 8 and 4. 5,respectively,greater than in control wild type（WT）. Besides,the expression of PRs in P. graminis infected plants 8 days later was higher than in non-infected plants. Defense mechanism of Kentucky bluegrass to rust was preliminary investigated and it presented the related disease-resistant gene and protein induced by rust pathogen. The research provides foundation for rust prevention and disease resistance breeding on Kentucky bluegrass in future.

关 键 词：草坪草 草地早熟禾 矮化突变材料 锈菌鉴定 抗病性 

分 类 号：S688.4[农业科学—观赏园艺]