双相脉冲电刺激对大鼠嗅球神经干细胞增殖及分化的影响  

Effect of biphasic electrical stimulation on the proliferation and differentiation of olfactory bulb neural precursor cells

作　　者：王梦航[1] 

机构地区：[1]北京大学国际医院耳鼻咽喉-头颈外科,北京102206

出　　处：《中华物理医学与康复杂志》2017年第3期161-164,共4页Chinese Journal of Physical Medicine and Rehabilitation

基　　金：国家自然科学基金科学基金项目（11202018）;中国博士后科学基金项目（20110490269,2013T60055）;北京大学国际医院院内科研基金（YN2016QN03）

摘　　要：目的观察双相脉冲电刺激对嗅球神经干细胞增殖及分化的影响。方法从新生1天的大鼠嗅球组织分离、培养、传代及鉴定嗅球神经干细胞；实验分为对照组和电刺激组。电刺激组利用双相脉冲电刺激细胞培养装置，使嗅球神经干细胞连续24h暴露于双相脉冲电刺激加载环境，脉冲宽度为8ms，并按脉冲强度的不同分为50mV组和100mV组；对照组：未暴露于电刺激加载环境。采用四甲基偶氮唑盐比色法（Mrrr法）和免疫荧光染色技术分别检测嗅球神经干细胞在双相脉冲电刺激下细胞增殖及分化的影响。结果50mV组及100mV组的细胞活力及GFAP阳性染色细胞数均较对照组明显增加，差异有统计学意义（P〈0．05）；50mv组明显高于100mv组（P〈0．05）。结论双相脉冲电刺激可促进嗅球神经干细胞的增殖及分化，因而有可能成为干细胞移植的一种辅助方法。Objective To explore the effect of biphasic electrical stimulation （BES） on the proliferation and differentiation of rats olfactory bulb neural precursor cells （ OB NPCs）, Methods OB NPCs of one day old neonatal rats were isolated, cultured, passaged and identified. They were randomly divided into a control group and an electrical stimulation （ES） group. The OB NPCs in the ES group were exposed to BES with a 50 mV/mm or 100 mV/mm electric field for ：24 hours with a pulse width of 8 ms, while the control group was not given electrical stimulation. The immunofluorescent technique and 3 （4, 5 dimethythiazol-2-yl ）-9, 5-diphenyl tetrazolium bromide （MTT） eolorimetric assay were used to assess the cells＇ proliferation and differentiation. Results The cell viabil- ity and the number of glial fibrillary acidic protein positive cellsin the ES group were significantly higher than in the control group. Conclusions BES may be used as an auxiliary technique for improving cell survival and differentiation in stem cell-based transplantation therapy as it can promote proliferation and differentiation, at least of OB NPCs.

关 键 词：电刺激 嗅球 神经干细胞 增殖 分化 神经损伤 

分 类 号：R245[医药卫生—针灸推拿学]