亚低温治疗窒息后新生儿脑损伤NF-κB和TNF-α的变化  

The changes of NF-κB and TNF-α levels in neonatal asphyxia with brain injury after mild hypothermia treatment

作　　者：王桂芳[1] 张玉[1] 王晓芳[1] 可秋萍[1] 吴焕卿[1] Wang Guifang Zhang Yu Wang Xiaofang Ke Qiuping Wu Huanqing(Department of Pediatrics, Xinxiang Central Hospital ,Xinxiang 453000, Chin)

机构地区：[1]河南新乡市中心医院儿科,新乡453000

出　　处：《中国实用神经疾病杂志》2017年第7期33-36,共4页Chinese Journal of Practical Nervous Diseases

摘　　要：目的观察窒息后新生儿脑损伤在亚低温治疗后NF-κB和TNF-α的变化,探讨亚低温治疗窒息后脑损伤的作用及机制。方法将符合亚低温入选条件55例窒息后脑损伤新生儿随机分成亚低温组28例与常规治疗组27例,同期正常产足月儿17例为对照组,亚低温组入院后即行亚低温联合三支持三对症治疗,常规治疗组给予三支持三对症治疗,分别于治疗前及治疗后24h、治疗后72h、生后7d检测血NF-κB和TNF-α水平。结果窒息组新生儿血清NF-κB和TNF-α水平较正常新生儿显著升高,亚低温治疗组的血清NF-κB和TNF-α水平低于常规治疗组(均P<0.01),亚低温治疗可降低窒息后脑损伤新生儿血清NF-κB和TNF-α水平。结论亚低温治疗窒息后脑损伤的作用机制可能与减少炎症介质NF-κB和TNF-α的表达,降低其含量有关。Objective To evaluate the changes of NF-κB and TNF-α levels in neonatal asphyxia with brain injury after mild hypothermia treatment,and study the effects and mechanism of mild hypothermia treatment.Methods Fifty-six newborns,who met the standard of mild hypothermia treatment,were randomly divided into the mild hypothermia treatment group and routine treatment group averagely.Meanwhile,normal control group included 17 newborn babies who were full term.The mild hypothermia treatment group was treated by mild hypothermia combining with the three supporting and symptomatic treatment after admission.In addition,the routine treatment group was treated by the three supporting and symptomatic treatment.The changes of NF-κB and TNF-α in serum were detected by ELISA method at 24 hand 72hafter treatment,7days after birth,respectively.Results Compared with the normal control group,the levels of NF-κB and TNF-α in serum of asphyxia neonatal group were significantly increased,and the levels of those in the mild hypothermia treatment group were lower than those the routine treatment group（both P〈0.01）.Conclusion The mechanism of mild hypothermia treatment may be related to reduce the expression and level of inflammatory mediators,such as NF-κB and TNF-α.

关 键 词：NF-ΚB TNF-Α 窒息 脑损伤 亚低温 新生儿 炎症介质 

分 类 号：R722.1[医药卫生—儿科]