非小细胞肺癌患者外周血CD4^+T淋巴细胞亚群的变化及临床意义  

Change of CD4^+ T lymphocyte subsets in patients with non-small cell lung cancer and its clinical significance

作　　者：殷红梅[1] 王磊[1] 胡晓波[1] 肖臻[2] 

机构地区：[1]上海中医药大学附属龙华医院检验科,上海200032 [2]上海中医药大学附属龙华医院儿科,上海200032

出　　处：《检验医学与临床》2017年第6期759-761,764,共4页Laboratory Medicine and Clinic

基　　金：上海市卫生局科研课题(20134266);上海市中医药事业发展三年行动计划(ZY3-CCCX-2-1002)

摘　　要：目的探讨非小细胞肺癌(NSCLC)患者外周血CD4^+T淋巴细胞亚群的变化及临床意义。方法选择2014年4月至2015年9月该院收治的68例NSCLC患者为研究对象(NSCLC组),以同期该院30例健康体检者为对照组,比较两组外周血辅助性T细胞1(Th1细胞)、Th2细胞、Th17细胞和调节性T细胞(Treg细胞)水平。同时对不同临床分期、不同病理分型、有无淋巴结转移情况和不同分化程度的NSCLC患者外周血T淋巴细胞亚群的变化进行分析。结果 (1)NSCLC组患者外周血中Th2细胞比例和Treg细胞比例显著高于对照组(P<0.05);而Th1细胞比例、Th1/Th2、Th17细胞比例和Th17/Treg均显著低于对照组(P<0.05)。(2)Ⅳ期患者外周血中Th2细胞比例和Treg细胞比例显著高于Ⅲ期(P<0.05);而Th1细胞比例、Th1/Th2、Th17细胞比例和Th17/Treg均显著低于Ⅲ期(P<0.05)。(3)鳞癌、无淋巴结转移或高分化的患者外周血中Treg细胞比例明显较低,Th17细胞比例和Th17/Treg则明显较高(P<0.05);而Th1、Th2细胞比例和Th1/Th2比较差异无统计学意义(P>0.05)。结论外周血Th1细胞、Th2细胞、Th17细胞和Treg细胞与NSCLC的发生和发展过程密切相关,尤其外周血Th17细胞和Treg细胞的检测对判断病情、了解机体的免疫功能具有重要的临床意义。Objective To investigate the change of peripheral blood CD4^＋T lymphocyte subsets in the patients with non-small cell lung cancer（NSCLC）and its clinical significance.Methods Sixty-eight cases of NSCLC in our hospital from April 2014 to September 2015 were selected as the research subjects and contemporaneous 30 persons undergoing physical examination were taken as the control group.The levels of Th1 cells,Th2cells,Th17 cells and Treg lymphocyte cells were examined between the two groups.Meanwhile the changes of peripheral blood T lymphocyte subsets in different clinical stages,different pathological types,whether having lymph node metastasis and different differentiation degrees were analyzed.Results（1）The proportions of peripheral blood Th2 cells and Treg cells in the NSCLC group were significantly higher than those in the control group（P〈0.05）,while the Th1 cells proportion,Th1/Th2,Th17 cells proportion and Th1/Treg were significantly lower than those in the control group（P〈0.05）.（2）The proportions of Th2 cells and Treg cells in the stageⅣ patients were significantly higher than those in the stageⅢpatients（P〈0.05）,while the Th1 cells proportion,Th1/Th2,Th17 cells proportion and Th17/Treg were significantly lower than those in the stageⅢ patients（P〈0.05）.（3）The Treg cells proportion in the patients with squamous cell carcinoma,no lymph node metastasis or high-differentiation was significantly lower（P〈0.05）,while the Th17 cells proportion and Th17/Treg were significantly higher（P〈0.05）;but the proportions of Th1 cells and Th2,and Th1/Th2 had no statistical difference（P〉0.05）.Conclusion Peripheral blood Th1 cells,Th2cells,Th17 cells and Treg cells are closely related with the development and progression of NSCLC,especially peripheral blood Th17 and Treg cells detection has an important clinical significance for judging the disease condition and understanding the body immune function.

关 键 词：非小细胞肺癌 T淋巴细胞亚群 CD4 

分 类 号：R725.622.5[医药卫生—儿科]