PSP-1-A的体外抗氧化研究  

Study on in vitro anti-oxidation of PSP-1-A

作　　者：李舒婕[1] 林海桢 彭雪雪[1] 冯焕村[1] 

机构地区：[1]南方医科大学第三附属医院,广州510665 [2]南方医科大学珠江医院,广州510282

出　　处：《检验医学与临床》2017年第6期776-777,780,共3页Laboratory Medicine and Clinic

摘　　要：目的对分离纯化得到的制黄精多糖PSP-1-A进行体外抗氧化活性测试。方法制黄精经分离纯化后得到制黄精粗多糖(PSP)及PSP-1-A,采用体外抗氧化实验法测定PSP及PSP-1-A清除2,2-二苯基-1-苦肼基(DPPH)自由基、对羟基自由基的能力及总抗氧化能力。结果测得PSP及PSP-1-A在体外均能明显清除DPPH自由基、对羟基自由基和ABTS+自由基,且其体外抗氧化功能在一定范围内随浓度的增大而增大。结论 PSP及PSP-1-A在一定浓度下均具有明显的体外抗氧化作用。Objective To research the in vitro antioxidative effect of separated and purified polysaccharide PSP-1-A from Polygonatum Sibiricum Redoute.Methods The polysacchrade PSP-1-A was obtained from Polygonatum Sibiricum Redoute by separation and purification.The in vitro antioxidative experimental method was adopted to determine the ability of PSP and PSP-1-A for clearing DPPH free radical and hydroxyl free radical,and total antioxidant capability.Results It was detected that PSP and PSP-1-A could obviously clear in vitro DPPH free radical,hydroxyl radical and ABTS＋radical,moreover its in vitro anti-oxidative effect was increased with its concentration increase in some range.Conclusion PSP and PSP-1-A have significant antioxidative activity in vitro under a certain concentration.

关 键 词：黄精 多糖 抗氧化 

分 类 号：R285.5[医药卫生—中药学]