白细胞介素10通过抑制自噬调节树突状细胞功能  

Interleukin-10 regulates functions of dendritic cell through autophagy inhibition

作　　者：屈玉兰 邓捷文 邓常文[1] 夏福灿[3] 郭振红[2] 白冲[1] QU Yu-Lan DENG Jie-Wen DENG Chang-Wen XIA Fu-Can GUO Zhen-Hong BAI Chong(Department of Respira- tory Critical Care Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, Chin)

机构地区：[1]第二军医大学长海医院呼吸与危重症医学科,上海200433 [2]第二军医大学医学免疫学国家重点实验室,上海200433 [3]浙江大学医学院免疫研究所,杭州310058

出　　处：《中国免疫学杂志》2017年第3期333-337,共5页Chinese Journal of Immunology

基　　金：国家自然科学基金面上项目(81270073;81570019)资助

摘　　要：目的:研究白细胞介素10(Interleukin-10,IL-10)对树突状细胞(Dendritic cells,DCs)自噬及功能的影响。方法:体外培养C57BL/6小鼠骨髓来源的DCs分为对照组、LPS刺激组、IL-10干预组、IL-10与雷帕霉素干预组、雷帕霉素干预组,分别通过流式细胞术分析DCs表面共刺激分子CD40、CD80的表达、DCs摄取OVA抗原的比例以及诱导OT2细胞增殖比例,ELISA检测DCs分泌IL-6,TNF-α的水平等DCs相关功能;蛋白免疫印迹法检测DCs自噬蛋白LC3的表达,比较组间差异,以探讨IL-10对DCs功能及自噬的影响。结果:(1)与LPS刺激组比较,IL-10处理组DCs的表面共刺激分子CD40、CD80的表达下降、分泌IL-6、TNF-α水平下降、刺激T细胞增殖的比例明显减弱、摄取OVA抗原的能力增加,IL-10+雷帕霉素干预组的DCs与IL-10单独处理组相比,表面CD80的表达明显增加(P<0.05)、分泌IL-6、TNF-α能力及刺激T细胞增殖的能力均明显增加(P<0.000 1)。(2)DCs的自噬相关蛋白(LC3Ⅱ/LC3Ⅰ比例)明显下降。结论:IL-10可能通过抑制DCs自噬水平调节树突状细胞功能。Objective： To study the mechanism of interleukin-10（ IL-10） inhibiting the function of dendritic cells（ DCs）. Methods： Cultured C57 BL /6 mouse bone marrow-derived DCs were divided into 5 groups： control group,LPS stimulated group,IL-10 treated group,IL-10＋Rapamycin treated group and Rapamycin treated group. The regulatory mechanism of IL-10 on dendritic cells were evaluated from DCs function,Flow cytometry was used to analyse the expression of DCs surface co-stimulator CD80,CD40 expression,the ability of uptaking antigen and stimulating T cell to proliferate; ELISA was used to detect the cytokines IL-6 and TNF-α. Western blot was used to analyse the autophagy related protein LC3. Compared the differences between the groups. Results：（ 1） Compared to LPS stimulated group,IL-10 treated group,DCs surface co-stimulator CD40,CD80 were decreased,IL-6 and TNF-α secretion level and the ability to stimulate T cell to proliferate were decreased,the ability to capture OVA antigen was increased. Compared to IL-10 treated group,the DCs surface co-stimulator CD80 was decreased（ P〈0. 05）,IL-6 and TNF-α secretion level and the ability to stimulate T cell to proliferate were increased（ P〈0. 000 1） in IL-10＋rapamycin treated group. In addition,autophagy related proteins LC3Ⅱ / LC3Ⅰ was decreased in IL-10 treated group. Conclusion： IL-10 may regulate functions of DCs through inhibiting the autophagy of DCs.

关 键 词：白细胞介素10 树突状细胞 自噬 调节功能 

分 类 号：R392.12[医药卫生—免疫学]