系统性红斑狼疮患者外周血单核细胞PD-L2分子的表达及其临床意义  

Expression and clinical significance of PD-L2 molecule on monocytes of peripheral blood in systemic lupus erythematosus

作　　者：吴萍萍[1] 沈冬 郭云娣[1] 张雅琴[1] 傅丰庆[3] 刘翠平[3] 孙静[1] WU Ping-Ping SHEN Dong GUO Yun-Di ZHANG Ya-Qin FU Feng-Qing LIU Cui-Ping SUN ring(Suzhou Vocational Health Technology, Suzhou Key Laboratory of Laboratory Medicine, Suzhou 215009, China)

机构地区：[1]苏州卫生职业技术学院苏州市检验医学重点实验室,苏州215009 [2]苏州科技城医院,苏州2151530 [3]苏州大学附属第一医院临床免疫研究所,苏州215007

出　　处：《中国免疫学杂志》2017年第3期414-417,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金资助项目(31170836);江苏省高校优秀科技创新团队项目(自身免疫病抗体的挖掘和应用)的资助

摘　　要：目的:分析系统性红斑狼疮患者外周血单核细胞PD-L2分子的表达及其与疾病活动程度的相关性。方法:收集26例系统性红斑狼疮患者及38例健康对照组外周血,Ficoll分离单个核细胞后,TRIZOL抽提总RNA,定量PCR分析PD-L2基因的表达,流式细胞仪检测CD14、PD-L2分子的表达。统计学分析其相关性及与疾病活动程度之间的关联。结果:定量PCR结果提示系统性红斑狼疮患者外周血单核细胞PD-L2基因表达高于健康对照组(P<0.05),流式发现单核细胞上PD-L2高表达(P<0.05),且活动期患者表达高于稳定期,并且与疾病的评分(SLEDAI)呈正相关(P<0.05)。结论:免疫分子PD-L2可能参与系统性红斑狼疮疾病的进程并可能成为疾病预防及治疗的潜在分子。Objective： To analyze PD-L2 expression on monocytes of peripheral blood cells in systemic lupus erythematosus（ SLE） and it’s correlation with the degree of disease activity. Methods： Peripheral blood of 26 cases of SLE patiens and 38 cases of healthy controls were collected. Peripheral blood mononuclear cells（ PBMC） were isolated and realtime PCR was carried on to analyze the PD-L2 gene expression. At the same time flow cytometry was performed to analyze the CD14 and PD-L2 expression. Results： PD-L2 was significantly up-regulated on monocytes in RA patients than in healthy controls and had correlation with the disease activity and the SLEAI score. Conclusion： These findings help to clarify the function of PD-L2,including its potential role as a biomarker for SLE.

关 键 词：系统性红斑狼疮 PD-L2 SLEDAI 

分 类 号：R392.11[医药卫生—免疫学]