创伤性脑损伤后海马区S1PR1表达与NSCs增殖的关系  

The association between hippocampal sphingosine-1-phosphate receptor 1 expression and neural stem cells proliferation following traumatic brain injury

作　　者：叶玉勤[1,2] 杨永祥[1] 苏鑫洪 何军[1] 陈晓燕[1] 贺晓生[1] YE Yuqin YANG Yongxiang SU Xinhong HE Jun CHEN Xiaoyan HE Xiaasheng(Department of Neurosurgery, Xijing Hospital, Fourth Military Medical University, Xi ＇an 710032 Department of Neurosurgery, 163rd Hospital of PIA, Changsha 4101300, China)

机构地区：[1]第四军医大学西京医院神经外科,陕西西安710032 [2]解放军第163医院神经外科,湖南长沙410000

出　　处：《中华神经外科疾病研究杂志》2017年第2期110-114,共5页Chinese Journal of Neurosurgical Disease Research

基　　金：国家自然科学基金资助项目(81171155)

摘　　要：目的探讨创伤性脑损伤(TBI)后大鼠海马区1-磷酸鞘氨醇受体1(S1PR1)的表达变化特点及其与神经干细胞(NSCs)增殖的关系。方法 96只健康雄性SD大鼠随机分为TBI后1、3、7、14、21、28 d组(n=10)和各时点相应的对照组(n=6)。采用控制性皮层损伤法建立大鼠TBI模型,5-溴脱氧尿嘧啶核苷(Brd U)和性别决定相关基因簇2(Sox2)免疫荧光染色双标NSCs,观察TBI后海马区NSCs的增殖趋势,Western Blot检测和分析海马区S1PR1的表达变化规律。结果与对照组相比,伤后第1天海马区NSCs数量增多,第7天达到高峰,第14、21、28天逐渐减少(P<0.05)。海马区S1PR1于伤后第1天表达显著上调,高峰出现在伤后第7天,于第14、21、28天表达逐渐下调(P<0.05)。结论 TBI后海马区S1PR1表达变化与NSCs增殖趋势在时程上一致,S1PR1可能在TBI后神经再生与修复过程中具有重要调控作用。Objective The potential association between hippecampal sphingosine-1-phosphate receptor 1 （S1PR1） expression and neural stem cells （NSCs） proliferation in a rat model of traumatic brain injury （TBI） was studied.Methods Ninety-six rats were randomly divided into TBI 1 d group, 3 d group, 7 d group, 14 d group, 21 d group, 28 d group （10 in each）, and six control groups （6 in each）. TBI model was induced by a controlled cortical injury device. NSCs were double-labeled by thymidine analog 5-Bromo-2-deoxyUridine （BrdU） and sex determining region Y-box 2 （Sox2） with inununofluorescence staining. The level of S1PR1 protein in hippocampus was detected by Western Blot at scheduled time-points after TBI. Results NSCs in hippocampus was activated at 1 d after TBI, reached the peak at 7 d, was followed by a decrease from 7 d to 28 d when maintained a higher level compared to control group （P 〈0.05）. Hippocampal S1PR1 protein was increased from 1 d post tramna, and peaked around 7 d, decreased at 14 d, 21 d, and28 d to a lower level but still higher than that of control group （P 〈0.05）. Conclusion The S1PR1 expression varies in a similar temporal pattern with NSCs proliferation in hippocampus, indicating that S1PR1 may be required for the neurogenesis after TBI.

关 键 词：创伤性脑损伤 1-磷酸鞘氨醇受体1 神经干细胞 海马 

分 类 号：R651.1[医药卫生—外科学]