蜂斗菜总内酯对动物高原缺氧保护作用的研究  

Protective effects of the total bakkenolides from Petasites. tricholobus on high altitude hypoxia

作　　者：王业晴 谢曜宇 张慧[2] 吴弘[3] 郭美丽[1] WANG Yeqing XIE Yaoyu ZHANG H ui WU Hong GUO Meili(Department of Pharmacognosy,School of Pharma cy, Second Military Medical University, Shanghai, 200433 China Department of Aerospace Medicine, Naval Medical Re search Institute, Shanghai, 200433 China Department of Cardiology, Changhai Hospital Affiliated to Second Military Med ical University, Shanghai, 200433 China)

机构地区：[1]第二军医大学药学院生药学教研室,上海200433 [2]海军医学研究所航空医学研究室,上海200433 [3]第二军医大学附属长海医院心内科,上海200433

出　　处：《药学实践杂志》2017年第2期116-120,125,共6页Journal of Pharmaceutical Practice

基　　金：国家科技重大专项(2009ZX09103-420)

摘　　要：目的探讨蜂斗菜总内酯对动物高原缺氧的保护作用。方法通过建立小鼠急性密闭缺氧模型、小鼠急性减压缺氧模型以及大鼠减压缺氧模型,观察蜂斗菜总内酯对实验动物存活时间及死亡率的影响,并检测动物血糖、不同脏器中糖原、三磷酸腺苷(adenosine triphosphate,ATP)、乳酸(lactic acid,LD)、乳酸脱氢酶(lactic dehydrogenase,LDH)的含量。结果蜂斗菜总内酯能够明显延长小鼠急性密闭缺氧的存活时间,提高存活时间增加率;能显著降低小鼠急性减压缺氧的死亡率;对于减压缺氧大鼠,能显著降低血糖含量,显著增加肝脏、骨骼肌、心肌中的糖原含量以及脑、肝脏、骨骼肌、心肌中的ATP含量,并能显著降低血浆、骨骼肌和心肌中的LD含量,降低心肌中的LDH含量。结论蜂斗菜总内酯对小鼠急性密闭缺氧、小鼠急性减压缺氧和大鼠减压缺氧均具有明显的保护作用,其抗高原缺氧作用与其降低血糖含量,提高脏器中糖原含量、ATP含量,降低脏器中LD及LDH含量有关。Objective To study the protective effects of the total bakkenolides from P. tricholobus on high altitude hypoxia. Method Normobaric hypoxia model and acute hypobarie hypoxia model in mice, hypobaric bypoxia model in rats were established for this study. Survival time and survival rate of mice were recorded. The level of blood sugar and glycogen, adeno sine triphosphate （ATP）, lactic acid （I.D）, lactic dehydrogenase （LDH） were detected in different organs of rats. Results The total bakkenolides significantly prolonged the survival time of mice in normobaric hypoxia model and reduced the death rate of mice in acute hypobaric hypoxia model. The total bakkenolides suppressed blood sugar level in rats and increased the glycogen level in rat liver, skeletal muscle and myocardium. It also elevated the ATP content in rat brain, liver, skeletal muscle and myocardium. Meanwhile, the content of LD in plasma, skeletal muscle, myocardium and LDH level in myocardium were reduced. Conclusion The total bakkenolides from P. tricholobus have protective effect on normobaric hypoxia model and acute hypobaric hypoxia model in mice as well as hypobaric hypoxia model in rats. Its anti hypoxia efficacy at high altitude may relate to the increased blood sugar, glycogen, and ATP level and reduced LD, LDH level in major organs.

关 键 词：蜂斗菜 蜂斗菜总内酯 高原 抗缺氧 减压缺氧 

分 类 号：R285.1[医药卫生—中药学]