高纯度单体制备及结构鉴定芹菜籽中3种苯酞成分  

High purity preparation and identification of three phthalide compounds from Apium graveolens

作　　者：陈雯慧[1] 沈刚[1] 陈海生[1] CHEN Wenhui SHEN Gang CHEN Haisheng(Department of Natural Medicinal Chemistry, School of Pharmacy, Second Military Medical University, Shanghai 200433, Chin)

机构地区：[1]第二军医大学药学院天然药物化学教研室,上海200433

出　　处：《药学实践杂志》2017年第2期138-140,共3页Journal of Pharmaceutical Practice

基　　金：上海市中药现代化专项资助(09dZ1974000)

摘　　要：目的分离和鉴定芹菜籽中苯酞类化合物。方法采用硅胶柱色谱、制备薄层色谱方法分离纯化苯酞类化学成分,用波谱分析技术鉴定化合物结构。结果从芹菜籽中分离制备得到3种主要的苯酞类化合物,分别鉴定为芹菜甲素、芹菜乙素和新蛇床内酯。结论采用该法制备的样品纯度大于98%,可作为对照品使用。Objective Isolation, purification and structure identification of phthalides from Apiurn graveolens L. Methods Silica gel column and preparative TLC chromatography were used to isolate and purify the phthalide compounds. The structures of those compounds were determined through spectral analysis. Results Three pure compounds were identified as 3 n-butylphthalide, sedanenolide and sedanolide. Conclusion Three pure phthalide compounds can be used as analytical reference standard for quantitative analysis of A. graveolens.

关 键 词：芹菜籽 芹菜甲素 芹菜乙素 新蛇床内酯 结构鉴定 

分 类 号：R284.2[医药卫生—中药学]