人脐带间充质干细胞联合戈舍瑞林对卵巢早衰的修复作用  

Effects of human umbilical cord mesenchymal stem cells combined with goserelin on repairing rat ovarian damage

作　　者：贾晓晨[1] 鲁振宇 孟文静[1] 史业辉[1] 

机构地区：[1]天津医科大学肿瘤医院国家肿瘤临床医学研究中心天津市肿瘤防治重点实验室天津市恶性肿瘤临床医学研究中心,300060 [2]天津普瑞赛尔生物科技有限公司

出　　处：《天津医药》2017年第1期25-29,I0003,共6页Tianjin Medical Journal

摘　　要：目的评价人脐带间充质干细胞(h UC-MSCs)联合戈舍瑞林对大鼠卵巢早衰模型的作用,并观察h UC-MSCs对化疗所致的损伤卵巢的趋化作用。方法环磷酰胺首次负荷剂量50 mg/kg后,以8 mg/(kg·d)的剂量连续腹腔注射14 d建立大鼠卵巢早衰模型。随机数字表法将120只SD大鼠分为A^E组,分别为正常组、生理盐水对照组、戈舍瑞林组、h UC-MSC组和h UC-MSC+戈舍瑞林组,各24只。B^E组通过腹腔注射环磷酰胺建立卵巢早衰模型。分批处死大鼠,测定血清雌二醇(E2)水平,计数初级和成熟卵泡数目。通过GFP转染MSCs,使用共聚焦显微镜检测通过尾静脉移植的h UC-MSCs在SD大鼠体内的分布和生长情况。动物活体成像系统观察h UC-MSCs在大鼠体内分布情况。结果第46天E组E2水平与A组相当,E组初始卵泡及成熟卵泡数与A组比较差异无统计学意义(P>0.05)。激光共聚焦显微镜下可见卵巢组织存在GFP阳性细胞的分布。结论 h UC-MSCs+戈舍瑞林组对卵巢损伤的修复效果较好,可使大鼠卵巢功能恢复到接近正常水平。Objective To observe the repairing effect of human umbilical cord mesenchymal stem cells（h UC-MSCs）and goserelin on chemotherapy-induced ovarian injury, and the distribution and growth of h UC-MSCs transplanted in ratchemotherapy-induced ovarian injury. Methods A total of 120 SD rats were randomized into group A-E： A normal group,B NS control group, C goserelin group, D h UC-MSCs group and E h UC-MSCs＋goserelin group. The rat premature ovarianfailure（POF） model was established by given a loading dose of cyclophosphamide（CTX, 50 mg/kg） followed by dailyintraperitoneal injection of CTX（8 mg/kg） for consecutive 14-day. The h UC-MSCs were injected through caudal vein, andgoserelin was given by subcutaneous injection 4 days before POF model established. The serum level of estrogen wasdetected and numbers of follicles were counted. After GFP was transfected by lentivirus, the distribution and growth of stemcells transplanted in rats were observed by animal in vivo imaging system. Results At day 46, the serum level of estrogenshowed no significant difference between group A and group E（P 〉 0.05）. There were no significant differences in thecounted follicles between group A and group E（P 〉 0.05）. After tail vein injection of the transfected cells, GFP positive cellswere found in injury ovarian. Conclusion There is a repairing effect of h UC-MSCs and goserelin on ovarian injury.

关 键 词：脐带 间质干细胞 戈舍瑞林 卵巢功能早衰 抗肿瘤联合化疗方案 

分 类 号：R711.75[医药卫生—妇产科学]