葡萄糖转运蛋白2在肝源性糖尿病患者肝组织中的表达及意义  

Expression of glucose transporter 2 in liver tissue in patients with hepatogenous diabetes and its significance

作　　者：何玉兰[1] 孙琳[2] 于红卫[3] 孟庆华[2] HE Yulan SUN Lin YU Hongwei et al(Department of Infectious Diseases, Beijing Pinggu District Hospital, Beijing 101200, China)

机构地区：[1]北京市平谷区医院感染科,北京101200 [2]首都医科大学附属北京佑安医院病理科,北京100069 [3]首都医科大学附属北京佑安医院重症肝病科,北京100069

出　　处：《临床肝胆病杂志》2017年第3期512-515,共4页Journal of Clinical Hepatology

摘　　要：目的探讨葡萄糖转运蛋白2(GLUT2)在肝源性糖尿病肝组织中的表达及意义。方法选取2012年6月-2014年7月首都医科大学附属北京佑安医院重症肝病科和肝胆外科肝组织标本40例,免疫组化检测各组(正常组、慢性乙型肝炎组、肝硬化组、肝源性糖尿病组)肝组织中GLUT2的表达水平。计量资料多组间比较采用单因素方差分析,两两比较采用LSD-t检验,计数资料比较采用Fisher确切概率法。结果肝源性糖尿病组的空腹血糖、糖化血红蛋白、糖化血清白蛋白、BMI以及肝组织脂肪变性比例与其他3组比较差异均有统计学意义(P值均<0.05)。免疫组化显示GLUT2主要表达于肝细胞膜,尤其是靠近窦腔部位的细胞膜上,在正常肝组织、慢性乙型肝炎、肝硬化肝组织的表达均表现为从中央静脉向汇管区逐渐减少的趋势,有1例在肝硬化肝细胞再生结节部位表达增强。肝源性糖尿病肝组织有明显的脂肪变性(6/10),GLUT2在脂变部位呈强阳性表达,且表达相对均匀,在脂变细胞周围肝细胞质中的表达也有所增加,与窦腔侧的表达无明显差别。结论肝源性糖尿病肝组织易合并脂肪变性,且GLUT2的表达明显增加,可能是其糖代谢异常的发病机制之一。Objective To investigate the immunohistochemical expression of glucose transporter 2（GLUT2） in patients with hepatogenous diabetes and its significance.Methods A total of 40 liver tissue samples were collected from patients who were treated in Department of Severe Liver Disease and Department of Hepatobiliary Surgery in Beijing You An Hospital,Capital Medical University,from June 2012 to July 2014.Immunohistochemistry was used to measure the expression of GLUT2 in liver tissue in all four groups（normal group,chronic hepatitis B group,liver cirrhosis group,and hepatogenous diabetes group）.A one-way analysis of variance was used for the comparison of continuous data between groups,and the least significant difference t-test was used for further comparison between any two groups;the Fisher＇s exact test was used for the comparison of categorical data between groups.Results There were significant differences in fasting blood glucose,glycosylated hemoglobin,glycosylated serum albumin,body mass index,and proportion of liver tissue with steatosis between the hepatogenous diabetes group and the other three groups（all P〈0.05）.Immunohistochemistry showed that GLUT2 was mainly expressed in the liver cell membrane,especially the cell membrane near the sinus cavity.In the patients with normal liver tissue,chronic hepatitis B,or liver cirrhosis,the expression of GLUT2 in liver tissue tended to gradually decrease from the central vein to the portal area,and one patient with liver cirrhosis had enhanced expression of GLUT2 in the regenerative nodules of hepatocytes.In the 10 patients with hepatogenous diabetes,6 had significant steatosis of the liver tissue and strongly positive and relatively even expression of GLUT2 at the site of steatosis,as well as the cytoplasm around steatotic hepatocytes,with no significant difference from the expression of GLUT2 in the sinus cavity.Conclusion Patients with hepatogenous diabetes tend to have steatosis in the liver tissue and a significant increase in the expression of GLUT

关 键 词：肝源性糖尿病 葡萄糖转运体2型 葡萄糖代谢障碍 

分 类 号：R587.1[医药卫生—内分泌]