辛伐他汀对白癜风氧化应激模型中角质形成细胞趋化因子分泌的影响  

Effects of simvastatin on the expression of chemokine in the keratinocyte under oxidative stress

作　　者：常毓倩 李舒丽[1] 坚哲[1] 安亚文[1] 高天文[1] 李春英[1] CHANG Yuqian LI Shuli JIAN Zhe AN Yawen GAO Tianwen LI Chunying.(Department of Dermatology,Xijing Hospital, Fourth Military Medical University,Xi＇an 710032, China)

机构地区：[1]第四军医大学西京皮肤医院,西安710032

出　　处：《中国麻风皮肤病杂志》2017年第2期79-82,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金资助项目(编号:XJZT15M10;81402599)

摘　　要：目的:明确辛伐他汀对氧化应激下人原代角质形成细胞(KC)分泌趋化因子CXCL9、CXCL10、CXCL11和CCL22的影响。方法:常规培养KC,H2O2组给予1 m M H2O2模拟白癜风KC氧化应激模型,实验组予不同浓度辛伐他汀(0.1μmol/L、0.5μmol/L、1.0μmol/L)预处理后加入H2O2;采用Real-time PCR、ELISA及Western blot检测CXCL9、CXCL10、CXCL11和CCL22的mRNA表达及蛋白分泌。结果:辛伐他汀组CXCL9、CXCL10、CXCL11水平低于H2O2组,CCL22水平高于且呈H2O2组,呈剂量依赖方式。结论:辛伐他汀能通过应激的角质形成细胞调控分泌Th1型趋化因子CXCL9、CXCL10、CXCL11及CCL22的水平。Objective： To determine the effect of simvastatin on the expression of CXCL9, CXCL10, CX- CLll and CCL22 in H202-treated primary human keratinocytes （KC）. Methods： KC in the experimental group was pre-treated with different concentration of simvastatin （0.1 μmol/L, 0.5 μmol/L and 1.0 μmol/ L）, and then, exposed to 1.0 mM H202 for 24 h. KC in the H202 group was treated with 1.0 mM H202 only. The expression levels of mRNA and protein of CXCL9, CXCL10, CXCLll and CCL22 were detected by Real -time PCR, Western blot and EL1SA. Results： The expression levels of mRNA and protein of CXCL9, CXCL10 and CXCL11 in the experimental group were higher than those in H202 group, and the level of CCL22 was lower than that in H2O2 group. Conclusion： Simvastatin can influence the levels of CXCLg, CXCLI0, CXCL11 and CCL22 through regulating oxidatively stressed KC.

关 键 词：辛伐他汀 原代角质形成细胞 氧化应激 趋化因子 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]