IL-6促进胰岛细胞胰岛素分泌在PCOS发病机制中的作用  

IL-6 induces insulin secretion in rat pancreatic islet cells in vitro

作　　者：洪岭[1] 伍园园[1] 唐传玲[1] 陈淼鑫[1] 赵美 滕晓明[1] 

机构地区：[1]同济大学附属第一妇婴保健院生殖医学中心,上海201204

出　　处：《同济大学学报（医学版）》2017年第1期40-43,共4页Journal of Tongji University(Medical Science)

摘　　要：目的探讨IL-6在多囊卵巢综合征(polycystic ovary syndrome,PCOS)高胰岛素血症和胰岛素抵抗中的作用。方法在体外培养的大鼠胰岛细胞培养液中加入10、100和1 000 pg/ml IL-6,于培养24、48、72 h后,采用放射免疫法检测IL-6对胰岛细胞胰岛素分泌的影响。结果培育48 h时,1 000 pg/ml IL-6板孔中的胰岛素浓度高于对照组,差异有统计学意义(P<0.05);培育72 h时,100、1 000 pg/ml IL-6板孔中的胰岛素浓度均高于对照组,差异有统计学意义(P<0.05)。结论 IL-6在体外具有刺激胰岛细胞分泌胰岛素的作用,这可能与PCOS患者常出现高胰岛素血症、胰岛素抵抗现象有关。Objective To investigate the effect of interlukin-6 （ IL-6 ） on insulin secretion in rat pancreatic islet cells in vitro. Methods Primarily cultured pancreatic islet cells were treated with IL-6 at concentration of 10, 100 and 1 000 pg/ml, respectively. The insulin contents in culture supernatant were detected by radioimmunoassay （RIA） at 24, 48 and 72 h after culture. Results The insulin levels in culture supernatant with IL-6 1 000 pg/ml at 48 h after culture were significantly higher than those in controls （ P 〈 0.05 ）. The insulin levels in supernatant with 1L-6 100 pg/ml and 1 000 pg/ml were significantly higher than those controls at 72 h after treatment. Conclusion IL-6 can induce insulin secretion in pancreatic islet cells in vitro, which may be associated with hyperinsulinemia and insulin resistance involved in polycystic ovarian syndrome.

关 键 词：白介素6 多囊卵巢综合征 胰岛素抵抗 胰岛 

分 类 号：R711.6[医药卫生—妇产科学]