路路通酸对乳腺癌MCF-7细胞和宫颈癌C-33A细胞增殖的影响  

Effect of betulonic acid on proliferation of human breast cancer MCF-7 cells and human cervical cancer C-33A cells

作　　者：穆晓婷[1] 钱平[1] 蒋璐璐[1] 王玥[1] 靳鑫[1] 张东方[1] 

机构地区：[1]中国医科大学药学院中药与生药学教研室,沈阳110122

出　　处：《实用药物与临床》2017年第3期254-257,共4页Practical Pharmacy and Clinical Remedies

摘　　要：目的研究路路通酸(BTA)对乳腺癌MCF-7细胞及宫颈癌C-33A细胞增殖的影响。方法采用MTT法检测不同浓度BTA作用不同时间后对MCF-7细胞、C-33A细胞的增殖抑制作用;流式细胞仪检测BTA处理后细胞周期的变化。结果 BTA作用于乳腺癌MCF-7细胞24、48、72 h后的IC50分别为(37.62±1.72)、(27.32±0.99)、(19.19±0.90)μmol/L。BTA作用于宫颈癌C-33A细胞24、48、72 h后的IC50分别为(34.55±0.88)、(27.20±1.03)、(16.74±0.79)μmol/L,BTA对2种细胞增殖有明显抑制作用,且呈浓度时间依赖性(P<0.05),流式结果显示,BTA将MCF-7细胞阻滞在S期,并诱导其凋亡;BTA将C-33A细胞阻滞在G1-S期。结论BTA对乳腺癌MCF-7细胞和宫颈癌C-33A细胞具有较强的增殖抑制作用,其机制与细胞周期阻滞和诱导细胞凋亡有关。Objective To study the effect of betulonic acid on proliferation of human breast cancer MCF-7cells and human cervical cancer C-33 A cells.Methods MTT assay was used to observe the effect of BTA on growth of cell MCF-7 and cell C-33 A in various concentrations for different time periods.Cell cycle and cell apoptosis of these cells were assessed by flow cytometry.Results MTT results showed that the IC_（50） on MCF-7 cells were（37.62±1.72）,（27.32±0.99） and（19.19±0.90） μmol/L after being cultured for 24 h,48 h,and 72 h,while on C-33 A cells they were（34.55±0.88）,（27.20±1.03） and（16.74±0.79） μmol/L.BTA could significantly inhibit the proliferation of MCF-7 cells and C-33 A cells in a dosage-and time-dependent manner（P0.05）.Flow cytometry analysis showed that BTA could induce MCF-7 cells arrest at S phase,and induce apoptosis,while it induced MCF-7 cells arrest at G_1-S phase.Conclusion BTA can inhibit the proliferation of MCF-7 cells and C-33 A cells;the change of cell cycle and apoptosis may be involved in the process of inhibition.

关 键 词：路路通酸 乳腺癌 宫颈癌 增殖抑制 

分 类 号：R285[医药卫生—中药学]