大片段PCR方法检测CYP2D6~* 5基因分型的选择与优化  

Selection and optimization of long-PCR method for CYP2D6~* 5 genotyping assay

作　　者：柴琳[1] 刘红[1] 许莉[1] 李一石[1] 

机构地区：[1]卫生部心血管药物临床研究重点实验室,中国医学科学院阜外医院,北京协和医学院,北京100037

出　　处：《实用药物与临床》2017年第3期307-311,共5页Practical Pharmacy and Clinical Remedies

基　　金：2010年国家临床重点专科建设项目--<卫生部重点实验室项目>;"重大新药创制"科技重大专项--<心血管创新药物临床研究技术平台建设>(2012ZX09303-008-001)

摘　　要：目的经过比较选择更优化的大片段PCR方法,应用到临床研究中对CYP2D6~* 5基因分型检测。方法选择两种大片段PCR方法(传统和巢式大片段PCR)进行比较优化,并对329例健康男性人群进行CYP2D6~* 5基因分型的检测。结果本研究中传统大片段PCR方法分型检测率为92.6%,巢式大片段PCR方法分型检测率为100%;在研究群体中CYP2D6~* 5等位基因的发生频率为3.9%(n=26)。结论优化的大片段PCR方法使CYP2D6~* 5基因分型更高效明确,且巢式大片段PCR方法优于传统大片段PCR方法。Objective To select a long-PCR method which is applied to the clinical research of CYP2D6~＊ 5genotyping.Methods Two long-PCR methods were selected,compared and optimized to detect the genotyping of CYP2D6~＊ 5 in 329 Chinese male subjects.Results In our study,successful detection rate of traditional long-PCR method was 92.6%,however the rate of nested long-PCR method was 100%and CYP2D6~＊ 5 allele frequency in our study was 3.9%（n=26）.Conclusion Optimization made these two long-PCR methods of CP2D6~＊ 5 more effective and accurate.Furthermore,the nested long-PCR is working better than the traditional one.

关 键 词：CYP2D6＊5 大片段PCR 巢式大片段PCR 基因分型 

分 类 号：R440[医药卫生—诊断学]