基因芯片技术对结核分枝杆菌耐药性检测的临床应用  

Clinical application of gene chip technology in the detection of drug resistance of Mycobacterium tuberculosis

作　　者：陈瑶[1] 吴英松[1] CHEN Yao WU Yingsong(School of Laboratory Medicine and Biotechnology, Southern Medical University, Guangzhou, Guangdong, China, 510515)

机构地区：[1]南方医科大学检验与生物技术学院,广东广州510515

出　　处：《分子诊断与治疗杂志》2017年第2期108-111,共4页Journal of Molecular Diagnostics and Therapy

基　　金：重大传染病创新检测试剂的研制和应用研究(广州市协同创新重大专项;201400000004-1)

摘　　要：目的评价基因芯片技术对结核分枝杆菌耐药性检测的效果。方法选取我院结核科2014年3月至2016年3月收治的涂片阳性的肺结核住院患者238例,取其痰标本,分别用基因芯片、传统的罗氏培养和药敏试验3种方法进行结核分枝杆菌的异烟肼耐药性检测和利福平耐药性检测;把罗氏培养和药敏试验的结果作为金标准,评价基因芯片技术的临床应用价值。结果基因芯片技术检测涂片阳性患者痰标本异烟肼耐药的情况与金标准无显著差异(P>0.05);利福平耐药的情况与金标准相比也无明显差异(P>0.05)。结论基因芯片能够快速、准确地检测结核分枝杆菌对利福平和异烟肼的耐药情况,是一种值得推广的临床实验室检测方法。Objective To evaluate the effect of gene chip technology on the drug resistance of Mycobacterium tuberculosis. Methods 238 patients with smear positive pulmonary tuberculosis admitted to our hospital from March 2014 to March 2016 were selected as the subjects. Three methods were used to detect isoniazid resistance and rifampicin resistance of Mycobacterium tuberculosisin sputum samples： gene chip,traditional Roche culture and a drug sensitivity test. The results of Roche culture and drug sensitivity test were used as the gold standard to evaluate the value of gene chip technology. Results There was no significant difference in the resistance rate of smear positive patients with isoniazid detected with gene chip and the gold standard（P〉0.05）. Similarly, there was no significant difference between the rifampin resistance rate determined by gene chip and the gold standard（P〉0.05）. Conclusion Gene chip technology can rapidly and accurately detect the resistance of Mycobacterium tuberculosis to rifampicin and isoniazid, and it is a valuable clinical laboratory diagnostic method.

关 键 词：基因芯片技术 结核分枝杆菌 耐药性 检测 

分 类 号：R440[医药卫生—诊断学]