野豌豆提取物抗氧化活性的研究  

Study on the antioxidant activity of extracts from Vicia sepium Linn.

作　　者：杨洁[1] 甘露珍[1] 张飞雪[1] 陈清杰[1] 吴宁华[1] 吴基良[1] YANG Jie GAN Luzhen ZHANG Feixue CHEN Qinjie WU Ninghua WU Jiliang(Hubei Provincial Key Laboratory of Cardiovascular, Cerebrovascular, and Metabolic Disorders, Hubei University of Science and Technology, Xianning, 437000 Chin)

机构地区：[1]湖北科技学院糖尿病心脑血管病变湖北省重点实验室,湖北咸宁437000

出　　处：《黑龙江畜牧兽医（下半月）》2017年第3期150-153,共4页

基　　金：国家自然科学基金项目(81270355);湖北科技学院糖尿病专项基金项目(14zx10)

摘　　要：为了研究野豌豆全草75%乙醇提取物的总黄酮、总多糖含量及体外化学抗氧化活性,试验采用氯化铝-醋酸钠(Al Cl3-Na Ac)体系和硫酸-苯酚方法测定提取物中的总黄酮、总多糖含量;还采用化学模拟体系测定其清除1,1-二苯基-2-三硝基苯肼自由基(DPPH·)和超氧阴离子自由基(O2-·)的能力以及总还原能力,探讨野豌豆提取物的体外抗氧化活性。结果表明:75%乙醇提取物的总黄酮、总多糖含量分别为3.69%和0.95%;其提取物清除DPPH·的能力与总还原能力较好,清除O2-·的能力(IC50=0.571 g/L)明显高于维生素C的清除活力(IC50=2.910 g/L)。说明野豌豆的75%乙醇提取物具有良好的体外抗氧化活性。To study the contents of total flavonoids and total polysaccharides and in vitro chemical antioxidant activity of 75% ethanol extracts from Vicia sepium Linn. , aluminum chloride - sodium acetate （ A1C13 - NaAc ） system and phenol - sulfuric acid method were used to deter- mined the contents of total flavonoids and total polysaccharides in the extracts. Furthermore, a chemical simulation system was used to determine the scavenging capacity of the extracts for the free - radicals of 1,1 - Diphenyl - 2 - picrylhydrazyl radical 2,2 - Diphenyl - 1 - （ 2,4,6 - trini- trophenyl） hydrazyl （DPPH） and superoxide anion and the total reducing capacity so as to investigate the in vitro antioxidant activity of the ex- tracts from Vicia sepium Linn. The results showed that the contents of total flavonoids and total polysaccharides of the 75% ethanol extracts were 3.69% and 0.95%, respectively. The extracts showed better capacity of scavenging the free radical of DPPH and better total reducing capaci- ty. However, the capacity of the extracts scavenging the free radical of superoxide anion （IC50 = 0. 571 0 g/L） was significantly higher than that of vitamin C （ IC50 = 2. 910 g/L）. The results indicate that the 75% ethanol extracts from Vicia sepium Linn. has good in vitro antioxidant activity.

关 键 词：中药材 抗氧化 1 1-二苯基-2-三硝基苯肼自由基 超氧阴离子自由基 二丁基羟基甲苯 

分 类 号：S542.3[农业科学—作物学] S853.75