HPLC法测定八味肉桂胶囊4种成分的含量  

Determination of effective constituents in Bawei Rougui capsules by HPLC

作　　者：杨秋霞[1] 徐方方 梁光焰[1] 吴云山 刘博[1,2] 果德安[2,3] 孟江[1] 

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]广州中医药大学第二附属医院/广东省中医科学院,广东广州510006 [3]中国科学院上海药物研究所,上海201203

出　　处：《广东药科大学学报》2017年第1期56-60,共5页Journal of Guangdong Pharmaceutical University

基　　金：国家自然科学基金项目(81202398);广东省科技计划项目(2013B010102006,2014A020221035,2015A020211025);广东省中医院中医药科学技术研究专项项目(YN2014ZHR209,YN2015MS03);国家中医药管理局临床基地科研专项项目(JDZX2015207)

摘　　要：目的建立同时测定八味肉桂胶囊中芍药苷、肉桂酸、胡椒碱、去氢木香内酯含量的方法。方法采用5C_(18)-MS-Ⅱ(4.6 mm×250 mm,5μm)色谱柱;流动相为乙腈-0.1%乙酸梯度洗脱;流速为1.0 m L/min;检测波长为230 nm;柱温为35℃。结果八味肉桂胶囊中芍药苷、肉桂酸、胡椒碱、去氢木香内酯能完全分离,质量浓度与其峰面积之间均呈良好的线性关系,相关系数均大于0.999。芍药苷平均回收率为98.26%,RSD为1.91%(n=6);肉桂酸平均回收率为100.54%,RSD为2.14%(n=6);胡椒碱平均回收率为100.46%,RSD为2.41%(n=6);去氢木香内酯平均回收率为98.42%,RSD为2.48%(n=6)。结论该法简便、准确,专属性强,可用于八味肉桂胶囊的质量控制。Objective To establish a simultaneous determination method for four effective materials in Bawei Rougui capsules. Methods The chromatographic method was carried out on a 5C18-MS-Ⅱ（ 4. 6 mm × 250 mm, 5 μm） column at temperature of 35 ℃ and the mobile phase was consisted of acetonitrile and 0.1%acetic acid with the flow rate of 1.0 mL/min. The UV detection wavelength was at 230 nm. Results In the quantitative analysis, four compounds were simultaneously separated completely. All the calibration curves and the regression equations showed good linear correlation between concentration and peak area with the correlation coefficients more than 0. 999. The average recovery results with relative standard deviation （ RSD） were listed as follows： paeoniflorin 98.26%（ RSD=1.91%, n=6） , cinnamic acid 100.54%（ RSD=2.14%, n=6） , piperine 100.46% （ RSD=2.41%, n=6） , dehydrocostus latone 98.42% （ RSD=2.48%, n=6） . Conclusion The HPLC method was validated to be specific, linear, precise and accurate, which could be used to for the quality control of Bawei Rougui capsules.

关 键 词：八味肉桂胶囊 芍药苷 肉桂酸 去氢木香内酯 胡椒碱 高效液相色谱法 

分 类 号：O657.72[理学—分析化学] R286.0[理学—化学]