萝卜硫素下调Traf6/NF-κB信号通路抑制肾癌细胞增殖的作用研究  

Inhibition of sulforaphane on the proliferation of kidney cancer RC-2 cells via Traf6/NF-κB pathway

作　　者：毛卫林[1] 田锦成 吴品[2] 刘艳[3] 

机构地区：[1]湖北民族学院附属民大医院泌尿外科,湖北恩施445000 [2]武汉大学人民医院第一临床学院,湖北武汉430060 [3]湖北民族学院附属民大医院检验科,湖北恩施445000

出　　处：《广东药科大学学报》2017年第1期127-130,共4页Journal of Guangdong Pharmaceutical University

摘　　要：目的探讨萝卜硫素对肾癌细胞(RC-2)增殖的影响及其机制。方法体外培养RC-2和Traf6过表达的RC-2细胞,不同浓度萝卜硫素处理对数生长期的细胞株,采用CCK8试剂盒检测萝卜硫素对RC-2细胞和过表达Traf6基因RC-2细胞增殖的抑制作用;免疫印迹(Western blot)和反转录聚合酶链反应(RT-PCR)方法检测RC-2细胞中Traf6和NF-κB蛋白及mRNA的表达水平。结果萝卜硫素的浓度大于10μmol/L时可抑制RC-2细胞的增殖,并随着浓度增加抑制率随之增强;Traf6的过表达可以促进RC-2细胞的增殖;RT-PCR和Western blot实验结果表明20μmol/L的萝卜硫素可以抑制Traf6的表达而引起NF-κB表达下调。结论萝卜硫素可以抑制RC-2细胞的增殖,其机制可能与下调Traf6/NF-κB信号通路蛋白表达有关。Objective To investigate the effect and mechanism of sulforaphane on the proliferation of kidney cancer RC-2 cells. Methods RC-2 cells and Traf6-overexpressed RC-2 cells were cultured in vitro. The cell proliferation was measured by CCK8 assay. The protein and mRNA expression of Traf6 and NF-κB were detected by western blot and RT-PCR. Results Treatment with over 10 μmol/L sulforaphane inhibited RC-2 cell proliferation in a dose-dependent way. The over-expression of Traf6 promoted the proliferation of RC-2 cells. Sulforaphane （ 20 μmol/L） inhibited downregulation of NF-κB induced by Traf6 expression. Conclusion Sulforaphane could suppress the proliferation of RC-2 cells via down-regulation of Traf6/NF-κB signaling.

关 键 词：萝卜硫素 肾癌细胞 增殖 肿瘤坏死因子受体相关分子6 细胞核因子-ΚB 

分 类 号：R285[医药卫生—中药学]