一种条带免疫印迹试剂用于HIV抗体确证的效果评价  被引量:9

Evaluation of the recomLine HIV-1 & HIV-2 IgG as a confirmatory assay

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作  者:王晓林[1] 梁姝[2] 孙国清[3] 刘永健[1] 常帅[1] 李敬云[1] 鲍作义[1] Wang Xiaolin Liang Shu Sun Guo-qing Liu Yongjian Chang Shuai Li Jingyun Bao Zuoyi(State Key Laboratory of Pathogen and Bi- osecurity, Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China Sichuan Center for Disease Control and Prevention Henan Center for Disease Control and Prevention)

机构地区:[1]军事医学科学院微生物流行病研究所病原微生物生物安全国家重点实验室,北京100071 [2]四川省疾病预防控制中心,成都610000 [3]河南省疾病预防控制中心,郑州450016

出  处:《中国艾滋病性病》2017年第1期63-64,68,共3页Chinese Journal of Aids & STD

摘  要:目的评估一种条带免疫印迹试剂确证艾滋病病毒(HIV)抗体的效果。方法用蛋白印迹试验(WB)和条带免疫印迹试剂(LIA)平行检测1 035份标本,按照各自的说明书判断结果,对检测结果进行比较和分析。结果总体上,两种试剂检测的一致率为95.3%,对经过确证的640份HIV抗体阳性标本(含2份HIV-1O组和2份HIV-2标本)的检测的结果完全一致。在339份WB试剂判断为阴性的标本中,LIA试剂有5份(1.5%)为不确定结果,而在362份LIA试剂判断为阴性的标本中,WB试剂有28份(7.7%)为不确定结果(χ~2=15.29,P<0.001)。在初次检测的341份标本中,确证了17份为HIV抗体阳性,考核试剂检测5份为不确定,12份为阳性。结论 LIA试剂显示出非特异反应暨不确定结果少的优势,可考虑用于HIV抗体的常规确证,并在实际应用中不断改进完善。Objective To evaluate the recomLine HIV-1 HIV-2IgG assay.Methods The performance of a recomLine HIV-1 HIV-2IgG immunoassay as a confirmatory test was evaluated compared with HIV Blot 2.2by parallel detection of 1035 plasma specimens.Results The agreement rate was 95.3% overall,and 100%to the results of 640 confirmatory HIV positive specimens,including 2specimens from HIV-1Ogroup and 2specimens from HIV-2.The indeterminate results of LIA assay was 5specimens(1.5%),while WB assay got 28 specimens of indeterminate results(7.7%),indicating that the rate of indeterminate results by LIA assay was significantly lower than that of WB assay(χ^2=15.29,P〈0.001).12 of 17HIV positive cases among 341 initial tested specimens were confirmed,and other 5specimens were tested as indeterminate by LIA assay.Conclusion The LIA assay shows obvious predominance on high specificity.The LIA assay should be applied and improved in the routine HIV confirmatory test.

关 键 词:艾滋病病毒抗体确证 蛋白印迹试验 条带免疫印迹试验 艾滋病病毒抗体不确定 

分 类 号:R373.9[医药卫生—病原生物学]

 

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