球形纳米银的合成及其与牛血清白蛋白的相互作用  

Synthesis of spherical silver nanoparticles and studies on the interactions with bovine serum albumin

作　　者：徐香玉[1] 武卫华[2] 杜中玉[3] 姜靓[1] 毛旭艳[1] 王浩[1] XU Xiangyu WU Weihua DU Zhongyu JIANG Liang MAO Xuyan WANG Hao(Bio-Nano ＆ Medical Engineering Institute, Jining Medical University, Jining 272067, China Affiliated Hospital of Jining Medical University, Jining 272067, China School of Basic Medicine Sciences, Jining Medical University, Jining 272067, China)

机构地区：[1]济宁医学院生物纳米技术与医学工程研究所,济宁272067 [2]济宁医学院附属医院,济宁272029 [3]济宁医学院基础医学院,济宁272067

出　　处：《济宁医学院学报》2017年第1期5-8,15,共5页Journal of Jining Medical University

基　　金：济宁医学院博士科研基金项目(JY14QD04;JY14QD01);山东省自然科学基金项目(ZR2016BP10)

摘　　要：目的研究球形纳米银与牛血清白蛋白相互作用的机理。方法利用化学还原法合成纳米银颗粒;利用扫描电子显微镜(SEM)对合成的银纳米颗粒进行形貌表征;并利用紫外可见吸收光谱法(UV-vis)和荧光光谱法(FL)研究了球形纳米银与牛血清白蛋白(BSA)在缓冲溶液中的结合反应。结果制得的纳米银颗粒为球形,其平均粒径约为25 nm,溶液颜色为黄色;当球形纳米银与BSA作用时,随着纳米银溶液浓度的增加,混合溶液的紫外吸收峰强度也随之增加,但荧光光谱则发生了猝灭。结论在溶液中球形纳米银与BSA可以自发结合发生反应并形成复合物。Objective To study the interaction mechanism of spherical Ag nanoparticles with bovine ser- um albumin （BSA）. Methods The silver nanoparticles were synthesized by the chemical reduction method. The morphology of Ag nanoparticles was performed using scanning electron microscopy （SEM）. The inter- action of spherical Ag nanoparticles with BSA in aqueous buffer solution has been investigated by ultraviolet visible absorption （UV-vis） and fluorescence spectroscopy. Results The shape of the silver nanoparticles was spherical. The mean diameter was 25 nm and the color of the solution was yellow. With the addition of Ag nanoparticles to BSA solution, the absorbance of the mixture solution was progressively increased, but the fluorescence intensity was decreased with increasing concentration of silver nanoparticles. Conclusion The analysis of the spectroscopical data indicates that the binding processes between Ag nanoparticles and BSA are spontaneous, and the Ag-BSA complexes can be formed in the solution.

关 键 词：牛血清白蛋白 球形纳米银 光谱法 

分 类 号：TB383[一般工业技术—材料科学与工程]