鸡HspHsp90α可溶性表达及与传染性法氏囊病病毒衣壳蛋白亲和力的鉴定  

Soluble Expression of Hsp90α and Its Affinity with Capsid Protein of Infectious Bursal Disease Virus

作　　者：高祥[1] 李辉[1] 陈玉明[1] 刘爱晶[1] 吴甜甜[1] 王永强[1] 高立[1] 高玉龙[1] 李凯[1] 刘长军[1] 崔红玉[1] 张艳萍[1] 潘青[1] 王笑梅[1,2] 祁小乐[1] GAO Xiang LI Hui CHEN Yuming LIU Aijing WU Tiantain WANG Yongqiang GAO Li GAO Yulong LI Kai LIU Changjun CUI Hongyu ZHANG Yanping PAN Qing WANG Xiaomei QI Xiaole(Division of Avian Infectious Diseases,State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute,Chinese Academy of Agriculture Sciences,Harbin,Heilongjiang 150001 Jiangsu Co-innovation Center for Prevention and Control of Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu Important 225009)

机构地区：[1]中国农业科学院哈尔滨兽医研究所,兽医生物技术国家重点实验室,禽传染病研究室,黑龙江哈尔滨150001 [2]江苏省动物重要疫病与人畜共患病协同创新中心,江苏扬州225009

出　　处：《中国家禽》2017年第5期17-21,共5页China Poultry

基　　金：国家自然科学基金重点项目(31430087);现代农业产业技术体系建设专项资金(CARS-42-G07)

摘　　要：热激蛋白90α(Heat shock protein 90,Hsp90α)在传染性法氏囊病病毒(Infectious bursal disease,IBDV)的致病过程中发挥重要作用。蛋白互作及其亲和力均可能导致不同的信号转导和生物效应,鸡源Hsp90α与IBDV衣壳蛋白VP2的互作已经被证明,但两者之间的亲和力尚未被鉴定。研究通过表达和纯化鸡的Hsp90α和IBDV超强毒株的VP2蛋白,运用表面等离子共振(Surface plasmon resonance,SPR)技术,揭示Hsp90α和IBDV VP2蛋白两者互作的动态过程,并确定其亲和力((KD)为7.105×10^(-8)M),为强相互作用。研究结果对进一步揭示IBDV的致病机制有重要意义。Heat shock protein 90 alpha（Hsp90α）plays important roles in the infection of infectious bursal disease virus（IBDV）. Interactions between proteins and their affinity may induce different signal transductions and biological effects,and the interaction between chicken Hsp90α and capsid protein VP2 of IBDV has been identified,but their affinity is unknown. To identify the affinity between Hsp90α and VP2 of very virulent IBDV,these two proteins were expressed and purified,and then their affinity was identified with surface plasmon resonance（SPR）. This study disclosed the dynamic process of Hsp90α-CK1α interaction,and the high affinity was identified（KD=7.105×10^-8M）,which would be beneficial for further understanding the pathogenic mechanism of IBDV.

关 键 词：HSP90Α 传染性法氏囊病病毒 衣壳蛋白 表面等离子共振 亲和力 

分 类 号：S855.3[农业科学—临床兽医学]