Ndrg2对成年大鼠皮层机械性损伤后神经发生的影响  

Involvement of Ndrg2 in the neurogenesis of adult rat cortex after traumatic injury

作　　者：张钦军[1,2] 师瑞[1] 石亚军[1,3] 郝明华[1,4] 史明[1] 赵钢[1] 

机构地区：[1]第四军医大学西京医院神经内科 [2]眉山心脑血管病医院神经内科 [3]解放军68310部队 [4]武警山东省总队医院

出　　处：《神经解剖学杂志》2017年第2期117-122,共6页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(81571223)

摘　　要：目的:探讨Ndrg2对成年大鼠皮层机械损伤后神经发生的影响及可能作用机制。方法:选取成年雄性Sprague-Dawley大鼠24只,利用立体定位法对大鼠皮层进行针刺机械损伤。随后分为4组(n=6):对照组(注射生理盐水)、病毒对照组(注射空载体病毒)、上调Ndrg2组(注射AAV-Ndrg2病毒)及抑制剂组(注射AAV-Ndrg2病毒和-分泌酶抑制剂DAPT)。术后7 d免疫荧光检测损伤区周边Ndrg2及Nestin表达变化。结果:在对照组和病毒对照组,针道周边的Ndrg2+细胞明显增多,并表达神经干细胞标记物Nestin;与病毒对照组相比,上调Ndrg2组的Ndrg2+及Ndrg2+/Nestin^+细胞进一步增多(P<0.01);注射Notch信号抑制剂DAPT后,与上调Ndrg2组相比,针道周边Nestin^+细胞数目明显减少(P<0.01),但Ndrg2+细胞数目无明显改变(P>0.05)。结论:大鼠皮层机械损伤后,Ndrg2可参与损伤区的神经发生过程;Notch信号通路可能在Ndrg2促神经发生中发挥关键作用。Objective：To study whether Ndrg2 is involved in the neurogenesis of adult rat cortex after traumatic injury,and to explore possible underlying mechanisms.Methods：Twenty-four male Sprague-Dawley rats were subjected to the stab injurywitha needle by a stereotaxic method,and then randomly divided into four groups：two control groups （control group and virus control group,which were administrated with saline and virus empty vector by intracerebroventricular injection （i.c.v.）,respectively）,and two experimental groups （Ndrg2 over-expression group and antagonist group,which were injected i.c.v.with AAV-Ndrg2 virus and AAV-Ndrg2 virus plus-secretase inhibitor DAPT,respectively）.Ndrg2-or Nestin-（a neural stem cell marker） expressing cells were detected in the boundary of lesion area by immunolluorescent stain at 7dafter stab injury.Results：In the control group and virus control group,the number of Ndrg2 ＋ cells at the lesion boundary was obviously increased,and these cells also expressed Nestin;Compared withthe virus control group,the numbers of Ndrg2 ＋ cells and Ndrg2 ＋/Nestin＋ cells were further increased （P 〈0.01） in the Ndrg2 overexpression group;After treated with Notch signaling antagonist DAPT,the number of Nestin ＋ cells was significantly decreased （P 〈 0.01）.However,DAPT did not affect the number of Ndrg2 ＋ cells （P 〉 0.05）.Conclusion：Ndrg2 could be involved in the cortical neurogenesis at the lesion boundary after injury,and Notch signaling pathway may play a key role in this process.

关 键 词：NDRG2 创伤性脑损伤 神经发生 NOTCH信号 大鼠 

分 类 号：R651.15[医药卫生—外科学]